Abstract
Radioautographic examination of skin fibroblasts grown in tissue culture from normal donors revealed heavy labeling of almost all cells following incubation with tritiated hypoxanthine. Cells from patients with Lesch-Nyhan's disease, lacking inosinate pyrophosphorylase, had only 10 grains or less per cell. When normal and abnormal cells were mixed prior to culture, there was a progressive increase, with culture time, in the percentage of heavily labeled cells so that by 96 hr, when the cells were confluent, over 95% of the cells were heavily labeled. Reduction of cell density by subculture produced a reversion to original values. Cultures from three obligatory heterozygotes revealed the expected mixed population of cells. This appears to be a practical approach to the identification of the heterozygote.
Original language | English (US) |
---|---|
Pages (from-to) | 609-615 |
Number of pages | 7 |
Journal | Biochemical Genetics |
Volume | 3 |
Issue number | 6 |
DOIs | |
State | Published - Dec 1969 |
ASJC Scopus subject areas
- Biochemistry
- Ecology, Evolution, Behavior and Systematics
- Molecular Biology
- Genetics