Cell surface immunoglobulin. VI. Dynamics on a human lymphoma line

I. Grundke Iqbal, J. W. Uhr

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Release of cell surface Ig and secretion of Ig was studied in Daudi cells, a human lymphoma cell line that synthesizes IgM and appears to be a neoplastic counterpart of B lymphocytes. In contrast to murine splenocytes, almost no internally labeled Ig and only a small proportion of cell surface Ig are released during 8 hr of in vitro incubation. Released cell surface Ig can be recovered in the medium predominantly as 8 S IgM monomer, probably attached noncovalently to a fragment of plasma membrane. This shedding of cell surface Ig appears to be dependent on the prior culture phase of the cells. Thus, in cells from cultures kept at high density, there is greater release of cell surface Ig, apparently associated with cell death.

Original languageEnglish (US)
Pages (from-to)159-163
Number of pages5
JournalEuropean Journal of Immunology
Volume4
Issue number3
StatePublished - 1974

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B-Cell Antigen Receptors
Lymphoma
Immunoglobulin M
Cell Culture Techniques
B-Lymphocytes
Cell Death
Cell Membrane
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Cell surface immunoglobulin. VI. Dynamics on a human lymphoma line. / Grundke Iqbal, I.; Uhr, J. W.

In: European Journal of Immunology, Vol. 4, No. 3, 1974, p. 159-163.

Research output: Contribution to journalArticle

Grundke Iqbal, I & Uhr, JW 1974, 'Cell surface immunoglobulin. VI. Dynamics on a human lymphoma line', European Journal of Immunology, vol. 4, no. 3, pp. 159-163.
Grundke Iqbal, I. ; Uhr, J. W. / Cell surface immunoglobulin. VI. Dynamics on a human lymphoma line. In: European Journal of Immunology. 1974 ; Vol. 4, No. 3. pp. 159-163.
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