Cell surface immunoglobulin. XII. Localization of immunoglobulin on murine lymphocytes by scanning immunoelectronmicroscopy

M. F. Lipscomb; K. V. Holmes; E. S. Vitetta; U. Hämmerling; J. W. Uhr

doi:10.1002/eji.1830050407

Cell surface immunoglobulin. XII. Localization of immunoglobulin on murine lymphocytes by scanning immunoelectronmicroscopy

M. F. Lipscomb, K. V. Holmes, E. S. Vitetta, U. Hämmerling, J. W. Uhr

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Abstract

The localization of immunoglobulin (Ig) on the surface of murine splenic lymphocytes has been studied with the scanning electron microscope (SEM), using a rabbit F(ab)₂ hybrid antibody directed against mouse Ig and tobacco mosaic virus (TMV) with TMV as a morphological marker. 35 % of spleen cells and 3 % of thymus cells were labeled by this method. Labeled cells were usually small lymphocytes with moderate numbers of microvilli. The Ig molecules were randomly distributed on the lymphocyte surface, appearing on microvilli as well as on the smooth surface of the cell.

Original language	English (US)
Pages (from-to)	255-259
Number of pages	5
Journal	European Journal of Immunology
Volume	5
Issue number	4
DOIs	https://doi.org/10.1002/eji.1830050407
State	Published - Apr 1975

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Cell surface immunoglobulin. XII. Localization of immunoglobulin on murine lymphocytes by scanning immunoelectronmicroscopy",

abstract = "The localization of immunoglobulin (Ig) on the surface of murine splenic lymphocytes has been studied with the scanning electron microscope (SEM), using a rabbit F(ab)2 hybrid antibody directed against mouse Ig and tobacco mosaic virus (TMV) with TMV as a morphological marker. 35 % of spleen cells and 3 % of thymus cells were labeled by this method. Labeled cells were usually small lymphocytes with moderate numbers of microvilli. The Ig molecules were randomly distributed on the lymphocyte surface, appearing on microvilli as well as on the smooth surface of the cell.",

author = "Lipscomb, {M. F.} and Holmes, {K. V.} and Vitetta, {E. S.} and U. H{\"a}mmerling and Uhr, {J. W.}",

year = "1975",

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language = "English (US)",

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AU - Lipscomb, M. F.

AU - Holmes, K. V.

AU - Vitetta, E. S.

AU - Hämmerling, U.

AU - Uhr, J. W.

PY - 1975/4

Y1 - 1975/4

N2 - The localization of immunoglobulin (Ig) on the surface of murine splenic lymphocytes has been studied with the scanning electron microscope (SEM), using a rabbit F(ab)2 hybrid antibody directed against mouse Ig and tobacco mosaic virus (TMV) with TMV as a morphological marker. 35 % of spleen cells and 3 % of thymus cells were labeled by this method. Labeled cells were usually small lymphocytes with moderate numbers of microvilli. The Ig molecules were randomly distributed on the lymphocyte surface, appearing on microvilli as well as on the smooth surface of the cell.

AB - The localization of immunoglobulin (Ig) on the surface of murine splenic lymphocytes has been studied with the scanning electron microscope (SEM), using a rabbit F(ab)2 hybrid antibody directed against mouse Ig and tobacco mosaic virus (TMV) with TMV as a morphological marker. 35 % of spleen cells and 3 % of thymus cells were labeled by this method. Labeled cells were usually small lymphocytes with moderate numbers of microvilli. The Ig molecules were randomly distributed on the lymphocyte surface, appearing on microvilli as well as on the smooth surface of the cell.

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JO - European Journal of Immunology

JF - European Journal of Immunology

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ER -

Cell surface immunoglobulin. XII. Localization of immunoglobulin on murine lymphocytes by scanning immunoelectronmicroscopy

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