Cell surface immunoglobulin. XVIII. Functional differences of B lymphocytes bearing different surface immunoglobulin isotypes

E. S. Vitetta, J. Forman, J. R. Kettman

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25 Citations (Scopus)

Abstract

Subclasses of B lymphocytes were exposed to lipopolysaccharide (LPS) and the proliferative, blastogenic, and polyclonal response of each subpopulation determined. The results indicate that 2 distinct subpopulations are responsible for the LPS induced response: cells bearing IgM or IgM plus IgD give rise to the polyclonal response and IgM secretion, whereas cells bearing IgD only proliferate and undergo blastogenesis. 3 populations of murine splenic B lymphocytes were characterized previously as those bearing only IgM, those bearing only IgD, and a population bearing both isotypes. These studies were designed to test the response of the IgM cells (IgM only or IgM plus IgD) vs. the IgD only cells to the B cell mitogen, LPS. Results suggest that after 1-4 days of culture, in the presence of mitogen, the IgM cells enlarge and elaborate an IgM polyclonal response.

Original languageEnglish (US)
Pages (from-to)1055-1066
Number of pages12
JournalJournal of Experimental Medicine
Volume143
Issue number5
StatePublished - 1976

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B-Cell Antigen Receptors
Immunoglobulin Isotypes
Immunoglobulin M
B-Lymphocytes
Immunoglobulin D
Lipopolysaccharides
Mitogens
Lymphocyte Activation
Population

ASJC Scopus subject areas

  • Immunology

Cite this

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AU - Forman, J.

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AB - Subclasses of B lymphocytes were exposed to lipopolysaccharide (LPS) and the proliferative, blastogenic, and polyclonal response of each subpopulation determined. The results indicate that 2 distinct subpopulations are responsible for the LPS induced response: cells bearing IgM or IgM plus IgD give rise to the polyclonal response and IgM secretion, whereas cells bearing IgD only proliferate and undergo blastogenesis. 3 populations of murine splenic B lymphocytes were characterized previously as those bearing only IgM, those bearing only IgD, and a population bearing both isotypes. These studies were designed to test the response of the IgM cells (IgM only or IgM plus IgD) vs. the IgD only cells to the B cell mitogen, LPS. Results suggest that after 1-4 days of culture, in the presence of mitogen, the IgM cells enlarge and elaborate an IgM polyclonal response.

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