Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein

Mark Siegelman, Martha W. Bond, W. Michael Gallatin, Tom St John, Harry T. Smith, Victor A. Fried, Irving L. Weissman

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

Partial amino acid sequence analysis of a purified lymphocyte homing receptor demonstrates the presence of two amino termini, one of which corresponds precisely to the amino terminus of ubiquitin. This observation extends the province of this conserved polypeptide to the cell surface and leads to a proposed model of the receptor complex as a core polypeptide modified by glycosylation and ubiquitination. Independent antibodies to ubiquitin serve to identify additional cell surface species, an indication that ubiquitination of cell surface proteins may be more general. It is proposed that functional binding of lymphocytes to lymph node high endothelial venules might involve the ubiquitinated region of the receptor; if true, cell surface ubiquitin could play a more general role in cell-cell interaction and adhesion.

Original languageEnglish (US)
Pages (from-to)823-829
Number of pages7
JournalScience
Volume231
Issue number4740
StatePublished - 1986

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Glycoproteins
Ubiquitin
Lymphocytes
Ubiquitination
Lymphocyte Homing Receptors
Peptides
Venules
Protein Sequence Analysis
Glycosylation
Cell Adhesion
Cell Communication
Membrane Proteins
Lymph Nodes
Antibodies

ASJC Scopus subject areas

  • General

Cite this

Siegelman, M., Bond, M. W., Gallatin, W. M., John, T. S., Smith, H. T., Fried, V. A., & Weissman, I. L. (1986). Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. Science, 231(4740), 823-829.

Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. / Siegelman, Mark; Bond, Martha W.; Gallatin, W. Michael; John, Tom St; Smith, Harry T.; Fried, Victor A.; Weissman, Irving L.

In: Science, Vol. 231, No. 4740, 1986, p. 823-829.

Research output: Contribution to journalArticle

Siegelman, M, Bond, MW, Gallatin, WM, John, TS, Smith, HT, Fried, VA & Weissman, IL 1986, 'Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein', Science, vol. 231, no. 4740, pp. 823-829.
Siegelman M, Bond MW, Gallatin WM, John TS, Smith HT, Fried VA et al. Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. Science. 1986;231(4740):823-829.
Siegelman, Mark ; Bond, Martha W. ; Gallatin, W. Michael ; John, Tom St ; Smith, Harry T. ; Fried, Victor A. ; Weissman, Irving L. / Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. In: Science. 1986 ; Vol. 231, No. 4740. pp. 823-829.
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