Cell-to-cell heterogeneity in growth rate and gene expression in Methylobacterium extorquens AM1

Tim J. Strovas, Linda M. Sauter, Xiaofeng Guo, Mary E. Lidstrom

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Cell-to-cell heterogeneity in gene expression and growth parameters was assessed in the facultative methylotroph Methylobacterium extorquens AM1. A transcriptional fusion between a well-characterized methylotrophy promoter (PmxaF) and gfpuv (encoding a variant of green fluorescent protein [GFPuv]) was used to assess single-cell gene expression. Using a flowthrough culture system and laser scanning microscopy, data on fluorescence and cell size were obtained over time through several growth cycles for cells grown on succinate or methanol. Cells were grown continuously with no discernible lag between divisions, and high cell-to-cell variability was observed for cell size at division (2.5-fold range), division time, and growth rate. When individual cells were followed over multiple division cycles, no direct correlation was observed between the growth rate before a division and the subsequent growth rate or between the cell size at division and the subsequent growth rate. The cell-to-cell variability for GFPuv fluorescence from the PmxaF promoter was less, with a range on the order of 1.5-fold. Fluorescence and growth rate were also followed during a carbon shift experiment, in which cells growing on succinate were shifted to methanol. Variability of the response was observed, and the growth rate at the time of the shift from succinate to methanol was a predictor of the response. Higher growth rates at the time of the substrate shift resulted in greater decreases in growth rates immediately after the shift, but full induction of P mxaF-gfPuv was achieved faster. These results demonstrate that in M. extorquens, physiological heterogeneity at the single-cell level plays an important role in determining the population response to the metabolic shift examined.

Original languageEnglish (US)
Pages (from-to)7127-7133
Number of pages7
JournalJournal of Bacteriology
Volume189
Issue number19
DOIs
StatePublished - Oct 2007

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Methylobacterium extorquens
Gene Expression
Growth
Succinic Acid
Cell Size
Methanol
Fluorescence
Green Fluorescent Proteins
Confocal Microscopy
Cell Division

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Cell-to-cell heterogeneity in growth rate and gene expression in Methylobacterium extorquens AM1. / Strovas, Tim J.; Sauter, Linda M.; Guo, Xiaofeng; Lidstrom, Mary E.

In: Journal of Bacteriology, Vol. 189, No. 19, 10.2007, p. 7127-7133.

Research output: Contribution to journalArticle

Strovas, Tim J. ; Sauter, Linda M. ; Guo, Xiaofeng ; Lidstrom, Mary E. / Cell-to-cell heterogeneity in growth rate and gene expression in Methylobacterium extorquens AM1. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 19. pp. 7127-7133.
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