Cell type and receptor identity regulate cholera toxin subunit B (CTB) internalization

Anirudh Sethi, Amberlyn M. Wands, Marcel Mettlen, Soumya Krishnamurthy, Han Wu, Jennifer J Kohler

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cholera toxin (CT) is a secreted bacterial toxin that binds to glycoconjugate receptors on the surface of mammalian cells, enters mammalian cells through endocytic mechanisms and intoxicates mammalian cells by activating cytosolic adenylate cyclase. CT recognizes cell surface receptors through its B subunit (CTB). While the ganglioside GM1 has been historically described as the sole receptor, CTB is also capable of binding to fucosylated glycoconjugates, and fucosylated molecules have been shown to play a functional role in host cell intoxication by CT. Here, we use colonic epithelial and respiratory epithelial cell lines to examine how two types of CT receptors—gangliosides and fucosylated glycoconjugates—contribute to CTB internalization. We show that fucosylated glycoconjugates contribute to CTB binding to and internalization into host cells, even when the ganglioside GM1 is present. The contributions of the two classes of receptors to CTB internalization depend on cell type. Additionally, in a cell line that harbours both classes of receptors, gangliosides dictate the efficiency of CTB internalization. Together, the results lend support to the idea that fucosylated glycoconjugates play a functional role in CTB internalization, and suggest that CT internalization depends on both receptor identity and cell type.

Original languageEnglish (US)
Article number20180076
JournalInterface Focus
Volume9
Issue number2
DOIs
StatePublished - Apr 6 2019

Fingerprint

Cholera Toxin
Cells
Glycoconjugates
Ports and harbors
G(M1) Ganglioside
Molecules
Bacterial Toxins
Cell Line
Cell Surface Receptors
Adenylyl Cyclases
Epithelial Cells

Keywords

  • Cholera
  • Exotoxin
  • Fucose
  • Glycosylation
  • GM1

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Cell type and receptor identity regulate cholera toxin subunit B (CTB) internalization. / Sethi, Anirudh; Wands, Amberlyn M.; Mettlen, Marcel; Krishnamurthy, Soumya; Wu, Han; Kohler, Jennifer J.

In: Interface Focus, Vol. 9, No. 2, 20180076, 06.04.2019.

Research output: Contribution to journalArticle

Sethi, Anirudh ; Wands, Amberlyn M. ; Mettlen, Marcel ; Krishnamurthy, Soumya ; Wu, Han ; Kohler, Jennifer J. / Cell type and receptor identity regulate cholera toxin subunit B (CTB) internalization. In: Interface Focus. 2019 ; Vol. 9, No. 2.
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