Cell type-specific activation of intracellular transglutaminase 2 by oxidative stress or ultraviolet irradiation: Implications of transglutaminase 2 in age-related cataractogenesis

Dong Myung Shin, Ju Hong Jeon, Chai Wan Kim, Sung Yup Cho, Joon Cheol Kwon, Hye Jin Lee, Kyung Ho Choi, Sang Chul Park, In Gyu Kim

Research output: Contribution to journalArticle

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Abstract

Transglutaminase (TGase) 2 is a ubiquitously expressed enzyme that modifies proteins by cross-linking or polyamination. An aberrant activity of TGase 2 has implicated its possible roles in a variety of diseases including age-related cataracts. However, the molecular mechanism by which TGase 2 is activated has not been elucidated. In this report, we showed that oxidative stress or UV irradiation elevates in situ TGase 2 activity. Neither the expression level nor the in vitro activity of TGase 2 appeared to correlate with the observed elevation of in situ TGase 2 activity. Screening a number of cell lines revealed that the level of TGase 2 activation depends on the cell type and also the environmental stress, suggesting that unrecognized cellular factor(s) may specifically regulate in situ TGase 2 activity. Concomitantly, we observed that human lens epithelial cells (HLE-B3) exhibited about 3-fold increase in in situ TGase 2 activity in response to the stresses. The activated TGase 2 catalyzed the formation of water-insoluble dimers or polymers of αB-crystallin, βB2-crystallin, and vimentin in HLE-B3 cells, providing evidence that TGase 2 may play a role in cataractogenesis. Thus, our findings indicate that in situ TGase 2 activity must be evaluated instead of in vitro activity to study the regulation mechanism and function of TGase 2 in biological and pathological processes.

Original languageEnglish (US)
Pages (from-to)15032-15039
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number15
DOIs
StatePublished - Apr 9 2004

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Oxidative stress
Oxidative Stress
Chemical activation
Irradiation
Crystallins
transglutaminase 2
Biological Phenomena
Vimentin
Pathologic Processes
Dimers
Cataract
Lenses
Screening
Polymers

ASJC Scopus subject areas

  • Biochemistry

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Cell type-specific activation of intracellular transglutaminase 2 by oxidative stress or ultraviolet irradiation : Implications of transglutaminase 2 in age-related cataractogenesis. / Shin, Dong Myung; Jeon, Ju Hong; Kim, Chai Wan; Cho, Sung Yup; Kwon, Joon Cheol; Lee, Hye Jin; Choi, Kyung Ho; Park, Sang Chul; Kim, In Gyu.

In: Journal of Biological Chemistry, Vol. 279, No. 15, 09.04.2004, p. 15032-15039.

Research output: Contribution to journalArticle

Shin, Dong Myung ; Jeon, Ju Hong ; Kim, Chai Wan ; Cho, Sung Yup ; Kwon, Joon Cheol ; Lee, Hye Jin ; Choi, Kyung Ho ; Park, Sang Chul ; Kim, In Gyu. / Cell type-specific activation of intracellular transglutaminase 2 by oxidative stress or ultraviolet irradiation : Implications of transglutaminase 2 in age-related cataractogenesis. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 15. pp. 15032-15039.
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