Cells treated with trinitrobenzene sulfonic acid express an antigenic determinant recognized by cytotoxic effector cells that is not detected on cells coated with trinitrophenylated proteins

R. Ciavarra, J. Forman

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Abstract

Spleen cells cultured with TNBS-treated stimulator cells or TNP-HGG generate H-2 restricted anti-TNP cytotoxic effector cells. Antigenic determinants recognized by anti-TNP effector cells were analyzed by a cold target competition assay. Three major observations emerged from these studies: 1) Anti-TNP effector cells sensitized against 1 mM TNBS-treated stimulator cells are blocked from killing TNBS-treated target cells by unlabeled TNBS-inhibitor cells but not inhibitor cells coated with TNP-HGG. However, the same effector cells are blocked from killing TNP-HGG-coated target cells by inhibitor cells treated either with TNBS or coated with TNP-HGG. 2) Inhibitor cells exposed to a low concentration of TNBS (0.01 mM) block effector cell activity in a manner similar to TNP-HGG-coated inhibitors. 3) Spleen cells sensitized against TNP-HGG are blocked from killing TNBS-treated target cells by both TNBS-treated and TNP-HGG-inhibitor cells. These results indicate that spleen cells sensitized against TNBS-treated stimulators generate effector cells against two different antigenic specificities; one is immunodominant and (1 mM) TNBS dependent, the second is not. Target cells treated with low concentrations of TNBS (0.01 mM) or coated with TNP-HGG express only the second determinant. It is possible that the (1 mM) TNBS-dependent immunodominant determinant represents TNP covalently coupled to H-2, whereas the second determinant consists of TNP-proteins noncovalently associated with H-2 molecules and may represent a cross-reaction with environmental antigens.

Original languageEnglish (US)
Pages (from-to)713-718
Number of pages6
JournalJournal of Immunology
Volume124
Issue number2
StatePublished - Jan 1 1980

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Trinitrobenzenes
Sulfonic Acids
Epitopes
Proteins
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Cells treated with trinitrobenzene sulfonic acid express an antigenic determinant recognized by cytotoxic effector cells that is not detected on cells coated with trinitrophenylated proteins",
abstract = "Spleen cells cultured with TNBS-treated stimulator cells or TNP-HGG generate H-2 restricted anti-TNP cytotoxic effector cells. Antigenic determinants recognized by anti-TNP effector cells were analyzed by a cold target competition assay. Three major observations emerged from these studies: 1) Anti-TNP effector cells sensitized against 1 mM TNBS-treated stimulator cells are blocked from killing TNBS-treated target cells by unlabeled TNBS-inhibitor cells but not inhibitor cells coated with TNP-HGG. However, the same effector cells are blocked from killing TNP-HGG-coated target cells by inhibitor cells treated either with TNBS or coated with TNP-HGG. 2) Inhibitor cells exposed to a low concentration of TNBS (0.01 mM) block effector cell activity in a manner similar to TNP-HGG-coated inhibitors. 3) Spleen cells sensitized against TNP-HGG are blocked from killing TNBS-treated target cells by both TNBS-treated and TNP-HGG-inhibitor cells. These results indicate that spleen cells sensitized against TNBS-treated stimulators generate effector cells against two different antigenic specificities; one is immunodominant and (1 mM) TNBS dependent, the second is not. Target cells treated with low concentrations of TNBS (0.01 mM) or coated with TNP-HGG express only the second determinant. It is possible that the (1 mM) TNBS-dependent immunodominant determinant represents TNP covalently coupled to H-2, whereas the second determinant consists of TNP-proteins noncovalently associated with H-2 molecules and may represent a cross-reaction with environmental antigens.",
author = "R. Ciavarra and J. Forman",
year = "1980",
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journal = "Journal of Immunology",
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T1 - Cells treated with trinitrobenzene sulfonic acid express an antigenic determinant recognized by cytotoxic effector cells that is not detected on cells coated with trinitrophenylated proteins

AU - Ciavarra, R.

AU - Forman, J.

PY - 1980/1/1

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N2 - Spleen cells cultured with TNBS-treated stimulator cells or TNP-HGG generate H-2 restricted anti-TNP cytotoxic effector cells. Antigenic determinants recognized by anti-TNP effector cells were analyzed by a cold target competition assay. Three major observations emerged from these studies: 1) Anti-TNP effector cells sensitized against 1 mM TNBS-treated stimulator cells are blocked from killing TNBS-treated target cells by unlabeled TNBS-inhibitor cells but not inhibitor cells coated with TNP-HGG. However, the same effector cells are blocked from killing TNP-HGG-coated target cells by inhibitor cells treated either with TNBS or coated with TNP-HGG. 2) Inhibitor cells exposed to a low concentration of TNBS (0.01 mM) block effector cell activity in a manner similar to TNP-HGG-coated inhibitors. 3) Spleen cells sensitized against TNP-HGG are blocked from killing TNBS-treated target cells by both TNBS-treated and TNP-HGG-inhibitor cells. These results indicate that spleen cells sensitized against TNBS-treated stimulators generate effector cells against two different antigenic specificities; one is immunodominant and (1 mM) TNBS dependent, the second is not. Target cells treated with low concentrations of TNBS (0.01 mM) or coated with TNP-HGG express only the second determinant. It is possible that the (1 mM) TNBS-dependent immunodominant determinant represents TNP covalently coupled to H-2, whereas the second determinant consists of TNP-proteins noncovalently associated with H-2 molecules and may represent a cross-reaction with environmental antigens.

AB - Spleen cells cultured with TNBS-treated stimulator cells or TNP-HGG generate H-2 restricted anti-TNP cytotoxic effector cells. Antigenic determinants recognized by anti-TNP effector cells were analyzed by a cold target competition assay. Three major observations emerged from these studies: 1) Anti-TNP effector cells sensitized against 1 mM TNBS-treated stimulator cells are blocked from killing TNBS-treated target cells by unlabeled TNBS-inhibitor cells but not inhibitor cells coated with TNP-HGG. However, the same effector cells are blocked from killing TNP-HGG-coated target cells by inhibitor cells treated either with TNBS or coated with TNP-HGG. 2) Inhibitor cells exposed to a low concentration of TNBS (0.01 mM) block effector cell activity in a manner similar to TNP-HGG-coated inhibitors. 3) Spleen cells sensitized against TNP-HGG are blocked from killing TNBS-treated target cells by both TNBS-treated and TNP-HGG-inhibitor cells. These results indicate that spleen cells sensitized against TNBS-treated stimulators generate effector cells against two different antigenic specificities; one is immunodominant and (1 mM) TNBS dependent, the second is not. Target cells treated with low concentrations of TNBS (0.01 mM) or coated with TNP-HGG express only the second determinant. It is possible that the (1 mM) TNBS-dependent immunodominant determinant represents TNP covalently coupled to H-2, whereas the second determinant consists of TNP-proteins noncovalently associated with H-2 molecules and may represent a cross-reaction with environmental antigens.

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