Cells treated with trinitrobenzene sulfonic acid express an antigenic determinant recognized by cytotoxic effector cells that is not detected on cells coated with trinitrophenylated proteins

R. Ciavarra, J. Forman

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Spleen cells cultured with TNBS-treated stimulator cells or TNP-HGG generate H-2 restricted anti-TNP cytotoxic effector cells. Antigenic determinants recognized by anti-TNP effector cells were analyzed by a cold target competition assay. Three major observations emerged from these studies: 1) Anti-TNP effector cells sensitized against 1 mM TNBS-treated stimulator cells are blocked from killing TNBS-treated target cells by unlabeled TNBS-inhibitor cells but not inhibitor cells coated with TNP-HGG. However, the same effector cells are blocked from killing TNP-HGG-coated target cells by inhibitor cells treated either with TNBS or coated with TNP-HGG. 2) Inhibitor cells exposed to a low concentration of TNBS (0.01 mM) block effector cell activity in a manner similar to TNP-HGG-coated inhibitors. 3) Spleen cells sensitized against TNP-HGG are blocked from killing TNBS-treated target cells by both TNBS-treated and TNP-HGG-inhibitor cells. These results indicate that spleen cells sensitized against TNBS-treated stimulators generate effector cells against two different antigenic specificities; one is immunodominant and (1 mM) TNBS dependent, the second is not. Target cells treated with low concentrations of TNBS (0.01 mM) or coated with TNP-HGG express only the second determinant. It is possible that the (1 mM) TNBS-dependent immunodominant determinant represents TNP covalently coupled to H-2, whereas the second determinant consists of TNP-proteins noncovalently associated with H-2 molecules and may represent a cross-reaction with environmental antigens.

Original languageEnglish (US)
Pages (from-to)713-718
Number of pages6
JournalJournal of Immunology
Volume124
Issue number2
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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