Cellular and molecular regulation of skeletal muscle side population cells

Annette P. Meeson, Thomas J. Hawke, Sarabeth Graham, Nan Jiang, Joel Elterman, Kelley Hutcheson, J. Michael DiMaio, Teresa D. Gallardo, Daniel J. Garry

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Muscle progenitor cells (satellite cells) function in the maintenance and repair of adult skeletal muscle. Side population (SP) cells are enriched in repopulating activity and also reside in adult skeletal muscle. In this study, we observed that Abcg2 is a determinant of the SP cell phenotype. Using reverse transcription polymerase chain reaction and immunohistochemical techniques, we localized Abcg2-expressing cells in the interstitium and in close approximation to the vasculature of adult skeletal muscle. Muscle SP cells are able to differentiate into myotubes and increase in number after cardiotoxin-induced muscle injury. Similar to myogenic progenitor cells, muscle SP cells express Foxk1 and are decreased in number in Foxk1 mutant skeletal muscle. Using emerging technologies, we examine the molecular signature of muscle SP cells from normal, injured, and Foxk1 mutant skeletal muscle to define common and distinct molecular programs. We propose that muscle SP cells are progenitor cells that participate in repair and regeneration of adult skeletal muscle.

Original languageEnglish (US)
Pages (from-to)1305-1320
Number of pages16
JournalStem Cells
Volume22
Issue number7
DOIs
StatePublished - 2004

Fingerprint

Side-Population Cells
Muscle Cells
Skeletal Muscle
Stem Cells
Cardiotoxins
Skeletal Muscle Fibers
Reverse Transcription
Regeneration
Maintenance
Technology
Phenotype
Muscles
Polymerase Chain Reaction
Wounds and Injuries

Keywords

  • Bone marrow
  • Foxk1
  • Myoblast
  • Side population cells
  • Skeletal muscle

ASJC Scopus subject areas

  • Cell Biology

Cite this

Meeson, A. P., Hawke, T. J., Graham, S., Jiang, N., Elterman, J., Hutcheson, K., ... Garry, D. J. (2004). Cellular and molecular regulation of skeletal muscle side population cells. Stem Cells, 22(7), 1305-1320. https://doi.org/10.1634/stemcells.2004-0077

Cellular and molecular regulation of skeletal muscle side population cells. / Meeson, Annette P.; Hawke, Thomas J.; Graham, Sarabeth; Jiang, Nan; Elterman, Joel; Hutcheson, Kelley; DiMaio, J. Michael; Gallardo, Teresa D.; Garry, Daniel J.

In: Stem Cells, Vol. 22, No. 7, 2004, p. 1305-1320.

Research output: Contribution to journalArticle

Meeson, AP, Hawke, TJ, Graham, S, Jiang, N, Elterman, J, Hutcheson, K, DiMaio, JM, Gallardo, TD & Garry, DJ 2004, 'Cellular and molecular regulation of skeletal muscle side population cells', Stem Cells, vol. 22, no. 7, pp. 1305-1320. https://doi.org/10.1634/stemcells.2004-0077
Meeson AP, Hawke TJ, Graham S, Jiang N, Elterman J, Hutcheson K et al. Cellular and molecular regulation of skeletal muscle side population cells. Stem Cells. 2004;22(7):1305-1320. https://doi.org/10.1634/stemcells.2004-0077
Meeson, Annette P. ; Hawke, Thomas J. ; Graham, Sarabeth ; Jiang, Nan ; Elterman, Joel ; Hutcheson, Kelley ; DiMaio, J. Michael ; Gallardo, Teresa D. ; Garry, Daniel J. / Cellular and molecular regulation of skeletal muscle side population cells. In: Stem Cells. 2004 ; Vol. 22, No. 7. pp. 1305-1320.
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