Cellular contact guidance through dynamic sensing of nanotopography

Meghan K. Driscoll, Xiaoyu Sun, Can Guven, John T. Fourkas, Wolfgang Losert

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We investigate the effects of surface nanotopography on the migration and cell shape dynamics of the amoeba Dictyostelium discoideum. Multiple prior studies have implicated the patterning of focal adhesions in contact guidance. However, we observe significant contact guidance of Dictyostelium along surfaces with nanoscale ridges or grooves, even though this organism lacks integrin-based adhesions. Cells that move parallel to nanoridges are faster, more protrusive at their fronts, and more elongated than are cells that move perpendicular to nanoridges. Quantitative studies show that nanoridges spaced 1.5 μm apart exhibit the greatest contact guidance efficiency. Because Dictyostelium cells exhibit oscillatory shape dynamics, we model contact guidance as a process in which stochastic cellular harmonic oscillators couple to the periodicity of the nanoridges. In support of this connection, we find that nanoridges nucleate actin polymerization waves of nanoscale width that propagate parallel to the nanoridges.

Original languageEnglish (US)
Pages (from-to)3546-3555
Number of pages10
JournalACS Nano
Volume8
Issue number4
DOIs
StatePublished - Apr 22 2014
Externally publishedYes

Fingerprint

Adhesion
cells
Integrins
Actins
Dynamic models
adhesion
amoeba
Polymerization
organisms
dynamic models
grooves
harmonic oscillators
ridges
periodic variations
polymerization

Keywords

  • cellular migration
  • cellular shape
  • Dictyostelium discoideum

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Cellular contact guidance through dynamic sensing of nanotopography. / Driscoll, Meghan K.; Sun, Xiaoyu; Guven, Can; Fourkas, John T.; Losert, Wolfgang.

In: ACS Nano, Vol. 8, No. 4, 22.04.2014, p. 3546-3555.

Research output: Contribution to journalArticle

Driscoll, MK, Sun, X, Guven, C, Fourkas, JT & Losert, W 2014, 'Cellular contact guidance through dynamic sensing of nanotopography', ACS Nano, vol. 8, no. 4, pp. 3546-3555. https://doi.org/10.1021/nn406637c
Driscoll, Meghan K. ; Sun, Xiaoyu ; Guven, Can ; Fourkas, John T. ; Losert, Wolfgang. / Cellular contact guidance through dynamic sensing of nanotopography. In: ACS Nano. 2014 ; Vol. 8, No. 4. pp. 3546-3555.
@article{ce7bbac6213d4cfd9bd135d79e89dfa4,
title = "Cellular contact guidance through dynamic sensing of nanotopography",
abstract = "We investigate the effects of surface nanotopography on the migration and cell shape dynamics of the amoeba Dictyostelium discoideum. Multiple prior studies have implicated the patterning of focal adhesions in contact guidance. However, we observe significant contact guidance of Dictyostelium along surfaces with nanoscale ridges or grooves, even though this organism lacks integrin-based adhesions. Cells that move parallel to nanoridges are faster, more protrusive at their fronts, and more elongated than are cells that move perpendicular to nanoridges. Quantitative studies show that nanoridges spaced 1.5 μm apart exhibit the greatest contact guidance efficiency. Because Dictyostelium cells exhibit oscillatory shape dynamics, we model contact guidance as a process in which stochastic cellular harmonic oscillators couple to the periodicity of the nanoridges. In support of this connection, we find that nanoridges nucleate actin polymerization waves of nanoscale width that propagate parallel to the nanoridges.",
keywords = "cellular migration, cellular shape, Dictyostelium discoideum",
author = "Driscoll, {Meghan K.} and Xiaoyu Sun and Can Guven and Fourkas, {John T.} and Wolfgang Losert",
year = "2014",
month = "4",
day = "22",
doi = "10.1021/nn406637c",
language = "English (US)",
volume = "8",
pages = "3546--3555",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "4",

}

TY - JOUR

T1 - Cellular contact guidance through dynamic sensing of nanotopography

AU - Driscoll, Meghan K.

AU - Sun, Xiaoyu

AU - Guven, Can

AU - Fourkas, John T.

AU - Losert, Wolfgang

PY - 2014/4/22

Y1 - 2014/4/22

N2 - We investigate the effects of surface nanotopography on the migration and cell shape dynamics of the amoeba Dictyostelium discoideum. Multiple prior studies have implicated the patterning of focal adhesions in contact guidance. However, we observe significant contact guidance of Dictyostelium along surfaces with nanoscale ridges or grooves, even though this organism lacks integrin-based adhesions. Cells that move parallel to nanoridges are faster, more protrusive at their fronts, and more elongated than are cells that move perpendicular to nanoridges. Quantitative studies show that nanoridges spaced 1.5 μm apart exhibit the greatest contact guidance efficiency. Because Dictyostelium cells exhibit oscillatory shape dynamics, we model contact guidance as a process in which stochastic cellular harmonic oscillators couple to the periodicity of the nanoridges. In support of this connection, we find that nanoridges nucleate actin polymerization waves of nanoscale width that propagate parallel to the nanoridges.

AB - We investigate the effects of surface nanotopography on the migration and cell shape dynamics of the amoeba Dictyostelium discoideum. Multiple prior studies have implicated the patterning of focal adhesions in contact guidance. However, we observe significant contact guidance of Dictyostelium along surfaces with nanoscale ridges or grooves, even though this organism lacks integrin-based adhesions. Cells that move parallel to nanoridges are faster, more protrusive at their fronts, and more elongated than are cells that move perpendicular to nanoridges. Quantitative studies show that nanoridges spaced 1.5 μm apart exhibit the greatest contact guidance efficiency. Because Dictyostelium cells exhibit oscillatory shape dynamics, we model contact guidance as a process in which stochastic cellular harmonic oscillators couple to the periodicity of the nanoridges. In support of this connection, we find that nanoridges nucleate actin polymerization waves of nanoscale width that propagate parallel to the nanoridges.

KW - cellular migration

KW - cellular shape

KW - Dictyostelium discoideum

UR - http://www.scopus.com/inward/record.url?scp=84899462468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899462468&partnerID=8YFLogxK

U2 - 10.1021/nn406637c

DO - 10.1021/nn406637c

M3 - Article

C2 - 24649900

AN - SCOPUS:84899462468

VL - 8

SP - 3546

EP - 3555

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 4

ER -