Cellular delivery of locked nucleic acids (LNAs).

Dwaine A. Braasch, David R. Corey

Research output: Contribution to journalArticle

Abstract

Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. They are synthesized using standard DNA/RNA synthesis methods, and have a negatively charged backbone that confers good solubility. This unit describes a method for the introduction of LNA oligomers into cells. A support protocol also describes the determination of melting temperatures for LNA oligomers.

Original languageEnglish (US)
Pages (from-to)Unit 4.13
JournalCurrent protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
VolumeChapter 4
StatePublished - Aug 2002

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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