Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. They are synthesized using standard DNA/RNA synthesis methods, and have a negatively charged backbone that confers good solubility. This unit describes a method for the introduction of LNA oligomers into cells. A support protocol also describes the determination of melting temperatures for LNA oligomers.
|Original language||English (US)|
|Pages (from-to)||Unit 4.13|
|Journal||Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]|
|State||Published - Aug 2002|
ASJC Scopus subject areas
- Organic Chemistry