Cellular delivery of locked nucleic acids (LNAs).

Dwaine A. Braasch; David R. Corey

Cellular delivery of locked nucleic acids (LNAs).

Research output: Contribution to journal › Article › peer-review

Abstract

Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. They are synthesized using standard DNA/RNA synthesis methods, and have a negatively charged backbone that confers good solubility. This unit describes a method for the introduction of LNA oligomers into cells. A support protocol also describes the determination of melting temperatures for LNA oligomers.

Original language	English (US)
Pages (from-to)	Unit 4.13
Journal	Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
Volume	Chapter 4
State	Published - Aug 2002

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

Cite this

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AB - Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. They are synthesized using standard DNA/RNA synthesis methods, and have a negatively charged backbone that confers good solubility. This unit describes a method for the introduction of LNA oligomers into cells. A support protocol also describes the determination of melting temperatures for LNA oligomers.

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Cellular delivery of locked nucleic acids (LNAs).

Abstract

ASJC Scopus subject areas

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