Cerebral microvessel endothelium is producing endothelin

Satonobu Yoshimoto, Yasuki Ishizaki, Hiroki Kurihara, Tomio Sasaki, Masao Yoshizumi, Masashi Yanagisawa, Yoshio Yazaki, Tomoh Masaki, Kintomo Takakura, Sei itsu Murota

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Endothelin, a potent vasoconstrictor peptide, was recently isolated from the supernatant of the cultured endothelia of the porcine aorta and is now supposed to be the most likely candidate for the endothelium-derived contractile factor (EDCF), which is responsible for the endothelium-dependent vasoconstriction by various stimuli. In this study, the production of endothelin by the endothelia of porcine cerebral microvessels was revealed by the Northern blot analysis with porcine endothelin cDNA probe and the enzyme-linked immunosorbent assay (ELISA) with anti-porcine endothelin antibody. Our results raise the possibility that the endothelia of cerebral microvessels regulate the local blood flow within the brain through the production of endothelin.

Original languageEnglish (US)
Pages (from-to)283-285
Number of pages3
JournalBrain Research
Volume508
Issue number2
DOIs
StatePublished - Feb 5 1990

Fingerprint

Endothelins
Microvessels
Endothelium
Swine
Vasoconstrictor Agents
Vasoconstriction
Northern Blotting
Aorta
Complementary DNA
Enzyme-Linked Immunosorbent Assay
Peptides
Antibodies
Brain

Keywords

  • Brain microvessel
  • Cell culture
  • Endothelial cell
  • Endothelin
  • Northern analysis

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Yoshimoto, S., Ishizaki, Y., Kurihara, H., Sasaki, T., Yoshizumi, M., Yanagisawa, M., ... Murota, S. I. (1990). Cerebral microvessel endothelium is producing endothelin. Brain Research, 508(2), 283-285. https://doi.org/10.1016/0006-8993(90)90407-3

Cerebral microvessel endothelium is producing endothelin. / Yoshimoto, Satonobu; Ishizaki, Yasuki; Kurihara, Hiroki; Sasaki, Tomio; Yoshizumi, Masao; Yanagisawa, Masashi; Yazaki, Yoshio; Masaki, Tomoh; Takakura, Kintomo; Murota, Sei itsu.

In: Brain Research, Vol. 508, No. 2, 05.02.1990, p. 283-285.

Research output: Contribution to journalArticle

Yoshimoto, S, Ishizaki, Y, Kurihara, H, Sasaki, T, Yoshizumi, M, Yanagisawa, M, Yazaki, Y, Masaki, T, Takakura, K & Murota, SI 1990, 'Cerebral microvessel endothelium is producing endothelin', Brain Research, vol. 508, no. 2, pp. 283-285. https://doi.org/10.1016/0006-8993(90)90407-3
Yoshimoto S, Ishizaki Y, Kurihara H, Sasaki T, Yoshizumi M, Yanagisawa M et al. Cerebral microvessel endothelium is producing endothelin. Brain Research. 1990 Feb 5;508(2):283-285. https://doi.org/10.1016/0006-8993(90)90407-3
Yoshimoto, Satonobu ; Ishizaki, Yasuki ; Kurihara, Hiroki ; Sasaki, Tomio ; Yoshizumi, Masao ; Yanagisawa, Masashi ; Yazaki, Yoshio ; Masaki, Tomoh ; Takakura, Kintomo ; Murota, Sei itsu. / Cerebral microvessel endothelium is producing endothelin. In: Brain Research. 1990 ; Vol. 508, No. 2. pp. 283-285.
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