Cerebrospinal fluid B cells from multiple sclerosis patients are subject to normal germinal center selection

Christopher Harp, Jane Lee, Doris L Washington, Elizabeth Cameron, Gregory Olsen, Elliot Frohman, Michael Racke, Nancy L Monson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Previous findings from our laboratory demonstrated that some clonally expanded cerebrospinal fluid (CSF) B cells from MS patients exhibit diminished mutation targeting patterns in comparison to typical B cells selected in the context of germinal centers (GCs). In order to determine whether the overall CSF B cell repertoires adhered to mutation patterns typical of GC-selected B cells, we analyzed the immunoglobulin repertoires from CSF B cells of 8 MS patients for mutation characteristics typical of GC-derived B cells. Mutation targeting was preserved. Thus, clonal expansion of some CSF B cells may occur independently of GC, but the CSF B cell pool is governed by typical GC selection. Interestingly, the heavy chain CDR3's of CSF B cells from MS patients had a net acidic charge, similar to GC-derived B cells, but a tendency towards longer CDR3's, consistent with autoreactive B cells. How these findings may support current hypotheses regarding the origin of CSF B cells is discussed.

Original languageEnglish (US)
Pages (from-to)189-199
Number of pages11
JournalJournal of Neuroimmunology
Volume183
Issue number1-2
DOIs
StatePublished - Feb 2007

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Germinal Center
Multiple Sclerosis
Cerebrospinal Fluid
B-Lymphocytes
Mutation
Immunoglobulins

Keywords

  • B lymphocytes
  • Cerebrospinal fluid
  • Immunoglobulin rearrangements
  • Multiple Sclerosis
  • Mutation accumulation

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Cerebrospinal fluid B cells from multiple sclerosis patients are subject to normal germinal center selection. / Harp, Christopher; Lee, Jane; Washington, Doris L; Cameron, Elizabeth; Olsen, Gregory; Frohman, Elliot; Racke, Michael; Monson, Nancy L.

In: Journal of Neuroimmunology, Vol. 183, No. 1-2, 02.2007, p. 189-199.

Research output: Contribution to journalArticle

Harp, Christopher ; Lee, Jane ; Washington, Doris L ; Cameron, Elizabeth ; Olsen, Gregory ; Frohman, Elliot ; Racke, Michael ; Monson, Nancy L. / Cerebrospinal fluid B cells from multiple sclerosis patients are subject to normal germinal center selection. In: Journal of Neuroimmunology. 2007 ; Vol. 183, No. 1-2. pp. 189-199.
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