Challenges for RNAi in vivo

Zain Paroo, David R. Corey

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Synthetic small interfering RNA (siRNA) has become a valuable tool for investigating gene function in cell culture. This success has led to high expectations for siRNA as a tool for in vivo investigation and as a platform for therapeutic development. siRNA in cell culture owes much of its success to years of development of traditional antisense oligonucleotides, and in vivo applications will also benefit from previous experience in this regard. However, the duplex nature of siRNA presents significant obstacles that will need to be overcome. Here, we discuss the current status of in vivo siRNA technology and describe some of the barriers to widespread application of RNAi-mediated gene silencing in mammals.

Original languageEnglish (US)
Pages (from-to)390-394
Number of pages5
JournalTrends in Biotechnology
Volume22
Issue number8
DOIs
StatePublished - Aug 1 2004

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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