Challenges for RNAi in vivo

Zain Paroo, David R. Corey

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Synthetic small interfering RNA (siRNA) has become a valuable tool for investigating gene function in cell culture. This success has led to high expectations for siRNA as a tool for in vivo investigation and as a platform for therapeutic development. siRNA in cell culture owes much of its success to years of development of traditional antisense oligonucleotides, and in vivo applications will also benefit from previous experience in this regard. However, the duplex nature of siRNA presents significant obstacles that will need to be overcome. Here, we discuss the current status of in vivo siRNA technology and describe some of the barriers to widespread application of RNAi-mediated gene silencing in mammals.

Original languageEnglish (US)
Pages (from-to)390-394
Number of pages5
JournalTrends in Biotechnology
Volume22
Issue number8
DOIs
StatePublished - Aug 1 2004

Fingerprint

RNA Interference
RNA
Small Interfering RNA
Cell culture
Cell Culture Techniques
Genes
Mammals
Antisense Oligonucleotides
Oligonucleotides
Gene Silencing
Technology
Therapeutics

ASJC Scopus subject areas

  • Bioengineering

Cite this

Challenges for RNAi in vivo. / Paroo, Zain; Corey, David R.

In: Trends in Biotechnology, Vol. 22, No. 8, 01.08.2004, p. 390-394.

Research output: Contribution to journalArticle

Paroo, Zain ; Corey, David R. / Challenges for RNAi in vivo. In: Trends in Biotechnology. 2004 ; Vol. 22, No. 8. pp. 390-394.
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