Changes in calmodulin main-chain dynamics upon ligand binding revealed by cross-correlated NMR relaxation measurements

Tianzhi Wang, Kendra King Frederick, Tatyana I. Igumenova, A. Joshua Wand, Erik R P Zuiderweg

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The fast dynamics of protein backbones are often investigated by nuclear magnetic relaxation experiments that report on the degree of spatial restriction of the amide bond vector. By comparing calmodulin in the peptide-bound and peptide-free states with these classical methods, we observe little difference in the dynamics of the polypeptide main chain (average order parameter decrease of 0.01 unit upon binding). However, when using NMR methods that monitor the mobility of the CO-Cα bond vector, we reveal a significant reduction of dynamics of the protein main chain (average order parameter decrease of 0.048 units). Previous investigations have suggested that the side-chain dynamics is reduced by an average of 0.07 order parameter units upon ligand binding (Lee, A. L.; Kinnear, S. A.; Wand, A. J. Nat. Struct. Biol. 2000, 7, 72-77). The current findings suggest that the change of the CO-Cα bond vector dynamics is intermediate between the changes in NH and side-chain dynamics and report a previously undetected loss of main-chain entropy. Weak site-to-site correlations between the different motional indicators are also observed.

Original languageEnglish (US)
Pages (from-to)828-829
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number3
DOIs
StatePublished - Jan 26 2005

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Calmodulin
Ligands
Nuclear magnetic resonance
Carbon Monoxide
Peptides
Entropy
Amides
Proteins
Magnetic relaxation
Polypeptides

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Changes in calmodulin main-chain dynamics upon ligand binding revealed by cross-correlated NMR relaxation measurements. / Wang, Tianzhi; Frederick, Kendra King; Igumenova, Tatyana I.; Wand, A. Joshua; Zuiderweg, Erik R P.

In: Journal of the American Chemical Society, Vol. 127, No. 3, 26.01.2005, p. 828-829.

Research output: Contribution to journalArticle

Wang, Tianzhi ; Frederick, Kendra King ; Igumenova, Tatyana I. ; Wand, A. Joshua ; Zuiderweg, Erik R P. / Changes in calmodulin main-chain dynamics upon ligand binding revealed by cross-correlated NMR relaxation measurements. In: Journal of the American Chemical Society. 2005 ; Vol. 127, No. 3. pp. 828-829.
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