TY - JOUR
T1 - Changes in chromatin proteins during liver regeneration
AU - Garrard, W. T.
AU - Bonner, J.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1974
Y1 - 1974
N2 - The metabolism of chromatin proteins of rat liver was examined during the prereplicative period following partial hepatectomy. Within 8 hr after partial hepatectomy, isolated chromatin exhibits a 7% decrease in its histone to DNA mass ratio compared to sham operated and zero time controls. The time course and amount of this decline correlate well with the previously published pattern of chromatin template activity increase. During the decrease in histone, the relative amounts of the 5 major species remain constant. However, there are differential losses and resyntheses of certain histone species. Histone I shows the most pronounced turnover, an estimated 12.5%. During regeneration, the non histone chromatin protein to DNA mass ratio does not change significantly, nor do the electrophoretic patterns of major non histone polypeptides. However, during the initial period of chromatin template activity increase (from 2 1/2 to 4 hr after the operation), the non histones as a group exhibit an increase in their rate of labeling greater than that of other subcellular fractions. Moreover, the rates of labeling of many non histone species become differentially stimulated, suggesting that their rates of loss from chromatin have also selectively increased.
AB - The metabolism of chromatin proteins of rat liver was examined during the prereplicative period following partial hepatectomy. Within 8 hr after partial hepatectomy, isolated chromatin exhibits a 7% decrease in its histone to DNA mass ratio compared to sham operated and zero time controls. The time course and amount of this decline correlate well with the previously published pattern of chromatin template activity increase. During the decrease in histone, the relative amounts of the 5 major species remain constant. However, there are differential losses and resyntheses of certain histone species. Histone I shows the most pronounced turnover, an estimated 12.5%. During regeneration, the non histone chromatin protein to DNA mass ratio does not change significantly, nor do the electrophoretic patterns of major non histone polypeptides. However, during the initial period of chromatin template activity increase (from 2 1/2 to 4 hr after the operation), the non histones as a group exhibit an increase in their rate of labeling greater than that of other subcellular fractions. Moreover, the rates of labeling of many non histone species become differentially stimulated, suggesting that their rates of loss from chromatin have also selectively increased.
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M3 - Article
C2 - 4369841
AN - SCOPUS:0016301447
SN - 0021-9258
VL - 249
SP - 5570
EP - 5579
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 17
ER -