Changes in chromatin proteins during liver regeneration

W. T. Garrard, J. Bonner

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The metabolism of chromatin proteins of rat liver was examined during the prereplicative period following partial hepatectomy. Within 8 hr after partial hepatectomy, isolated chromatin exhibits a 7% decrease in its histone to DNA mass ratio compared to sham operated and zero time controls. The time course and amount of this decline correlate well with the previously published pattern of chromatin template activity increase. During the decrease in histone, the relative amounts of the 5 major species remain constant. However, there are differential losses and resyntheses of certain histone species. Histone I shows the most pronounced turnover, an estimated 12.5%. During regeneration, the non histone chromatin protein to DNA mass ratio does not change significantly, nor do the electrophoretic patterns of major non histone polypeptides. However, during the initial period of chromatin template activity increase (from 2 1/2 to 4 hr after the operation), the non histones as a group exhibit an increase in their rate of labeling greater than that of other subcellular fractions. Moreover, the rates of labeling of many non histone species become differentially stimulated, suggesting that their rates of loss from chromatin have also selectively increased.

Original languageEnglish (US)
Pages (from-to)5570-5579
Number of pages10
JournalJournal of Biological Chemistry
Volume249
Issue number17
StatePublished - 1974

Fingerprint

Liver Regeneration
Liver
Histones
Chromatin
Proteins
Hepatectomy
Labeling
Subcellular Fractions
DNA
Metabolism
Rats
Regeneration
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Garrard, W. T., & Bonner, J. (1974). Changes in chromatin proteins during liver regeneration. Journal of Biological Chemistry, 249(17), 5570-5579.

Changes in chromatin proteins during liver regeneration. / Garrard, W. T.; Bonner, J.

In: Journal of Biological Chemistry, Vol. 249, No. 17, 1974, p. 5570-5579.

Research output: Contribution to journalArticle

Garrard, WT & Bonner, J 1974, 'Changes in chromatin proteins during liver regeneration', Journal of Biological Chemistry, vol. 249, no. 17, pp. 5570-5579.
Garrard, W. T. ; Bonner, J. / Changes in chromatin proteins during liver regeneration. In: Journal of Biological Chemistry. 1974 ; Vol. 249, No. 17. pp. 5570-5579.
@article{7551b2840ae044edaaf92d937dd4fec1,
title = "Changes in chromatin proteins during liver regeneration",
abstract = "The metabolism of chromatin proteins of rat liver was examined during the prereplicative period following partial hepatectomy. Within 8 hr after partial hepatectomy, isolated chromatin exhibits a 7{\%} decrease in its histone to DNA mass ratio compared to sham operated and zero time controls. The time course and amount of this decline correlate well with the previously published pattern of chromatin template activity increase. During the decrease in histone, the relative amounts of the 5 major species remain constant. However, there are differential losses and resyntheses of certain histone species. Histone I shows the most pronounced turnover, an estimated 12.5{\%}. During regeneration, the non histone chromatin protein to DNA mass ratio does not change significantly, nor do the electrophoretic patterns of major non histone polypeptides. However, during the initial period of chromatin template activity increase (from 2 1/2 to 4 hr after the operation), the non histones as a group exhibit an increase in their rate of labeling greater than that of other subcellular fractions. Moreover, the rates of labeling of many non histone species become differentially stimulated, suggesting that their rates of loss from chromatin have also selectively increased.",
author = "Garrard, {W. T.} and J. Bonner",
year = "1974",
language = "English (US)",
volume = "249",
pages = "5570--5579",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "17",

}

TY - JOUR

T1 - Changes in chromatin proteins during liver regeneration

AU - Garrard, W. T.

AU - Bonner, J.

PY - 1974

Y1 - 1974

N2 - The metabolism of chromatin proteins of rat liver was examined during the prereplicative period following partial hepatectomy. Within 8 hr after partial hepatectomy, isolated chromatin exhibits a 7% decrease in its histone to DNA mass ratio compared to sham operated and zero time controls. The time course and amount of this decline correlate well with the previously published pattern of chromatin template activity increase. During the decrease in histone, the relative amounts of the 5 major species remain constant. However, there are differential losses and resyntheses of certain histone species. Histone I shows the most pronounced turnover, an estimated 12.5%. During regeneration, the non histone chromatin protein to DNA mass ratio does not change significantly, nor do the electrophoretic patterns of major non histone polypeptides. However, during the initial period of chromatin template activity increase (from 2 1/2 to 4 hr after the operation), the non histones as a group exhibit an increase in their rate of labeling greater than that of other subcellular fractions. Moreover, the rates of labeling of many non histone species become differentially stimulated, suggesting that their rates of loss from chromatin have also selectively increased.

AB - The metabolism of chromatin proteins of rat liver was examined during the prereplicative period following partial hepatectomy. Within 8 hr after partial hepatectomy, isolated chromatin exhibits a 7% decrease in its histone to DNA mass ratio compared to sham operated and zero time controls. The time course and amount of this decline correlate well with the previously published pattern of chromatin template activity increase. During the decrease in histone, the relative amounts of the 5 major species remain constant. However, there are differential losses and resyntheses of certain histone species. Histone I shows the most pronounced turnover, an estimated 12.5%. During regeneration, the non histone chromatin protein to DNA mass ratio does not change significantly, nor do the electrophoretic patterns of major non histone polypeptides. However, during the initial period of chromatin template activity increase (from 2 1/2 to 4 hr after the operation), the non histones as a group exhibit an increase in their rate of labeling greater than that of other subcellular fractions. Moreover, the rates of labeling of many non histone species become differentially stimulated, suggesting that their rates of loss from chromatin have also selectively increased.

UR - http://www.scopus.com/inward/record.url?scp=0016301447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016301447&partnerID=8YFLogxK

M3 - Article

VL - 249

SP - 5570

EP - 5579

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 17

ER -