Changes in expression of CD45R during the development of Th1 and Th2 cell lines

William T. Lee, Ellen S. Vitetta

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Previous studies using anti-CD45R monoclonal antibodies (mAb) have shown that normal CD4+ T cells can be separated into virgin and memory cells based on their level of expression of CD45R. CD45Rhi (virgin) T cells secrete interleukin (IL)-2 whereas CD45Rlo (memory) Tcells secrete both IL-2 and IL-4. In contrast to these results studies using cultured T cell lines have shown that IL-2-secreting T helper cell type 1 (Th1) cells are CD45Rlo and IL-4-secreting Th2 cells are CD45Rhi. To resolve this discrepancy, and to determine how the Th1 and Th2 cell lines relate to memory CD45Rlo and virgin CD45Rhi CD4+ T cells, we have isolated CD45Rlo cells from alloantigen-primed mice and developed allospecitic Th1 and Th2 cell lines. The lymphokines secreted by these lines were then evaluated. Our results show that as CD45Rlo T cells develop into in vitro cell lines which secrete IL-4 they become CD45Rhi. In contrast, CD45Rlo cells that develop into lines which secrete IL-2 maintain their CD45Rlo phenotype. Thus, although both Th1 and Th2 cells arise initially from CD45Rlo cells, Th2 cells become CD45Rhi during prolonged in vitro culture but, Th1 cells do not.

Original languageEnglish (US)
Pages (from-to)1455-1459
Number of pages5
JournalEuropean Journal of Immunology
Volume22
Issue number6
StatePublished - Jun 1992

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Th2 Cells
Th1 Cells
Cell Line
Interleukin-2
T-Lymphocytes
Interleukin-4
Isoantigens
Lymphokines
Cultured Cells
Monoclonal Antibodies
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Changes in expression of CD45R during the development of Th1 and Th2 cell lines. / Lee, William T.; Vitetta, Ellen S.

In: European Journal of Immunology, Vol. 22, No. 6, 06.1992, p. 1455-1459.

Research output: Contribution to journalArticle

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