Changes in RNA Synthesis and Messenger RNA Content in the Cerebellum of Rats with Graft Versus Host Disease

W. S T Griffin, J. R. Head, S. Pardue, M. R. Morrison

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Cerebellar RNA accumulation, synthesis, and functional capacity was studied in 14‐day‐old F, hybrid rats subjected to neonatally induced graft versus host disease (GVHD). There was a decrease in RNA synthetic rate as measured by the uptake of labeled precursors into RNA. The decrease in total cerebellar RNA synthesis was reflected both in a reduced amount of Nissl substance, visible in cresyl violet‐stained 10‐μ‐thick sections of cerebella, and in the total amount of cytoplasmic RNA isolated from individual cerebella from diseased animals compared with control littermates. Analysis of the RNA translational capacity in wheat germ protein synthesizing systems showed that RNA from experimental animals was also biologically less active. Qualitative differences between protein populations in control and diseased animals were analyzed by two‐dimensional gel electrophoresis. There were few alterations in the steady state levels of cerebellar protein. However, two‐dimensional gel electrophoresis of the peptides synthesized in vitro by RNA from control and diseased animals showed that there were several changes in the relative abundance of some mRNAs between the two RNA populations. These data show that the cerebellar RNA from rats with GVHD differs both qualitatively and quantitatively from that of controls.

Original languageEnglish (US)
Pages (from-to)880-888
Number of pages9
JournalJournal of Neurochemistry
Volume35
Issue number4
DOIs
StatePublished - Oct 1980

Keywords

  • RNA synthesis—Messenger RNA—Cerebellum—Graft‐versus‐host disease. Griffin W. S. T. et al. Changes in RNA synthesis and messenger RNA content in the cerebellum of rats with graft versus host disease. J. Neurochem.35, 880–888 (1980)

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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