Changes in rRNA transcription influence proliferation and cell fate within a stem cell lineage

Qiao Zhang, Nevine A. Shalaby, Michael Buszczak

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Ribosome biogenesis drives cell growth and proliferation, but mechanisms that modulate this process within specific lineages remain poorly understood. Here, we identify a Drosophila RNA polymerase I (Pol I) regulatory complex composed of Under-developed (Udd), TAF1B, and a TAF1C-like factor. Disruption of udd or TAF1B results in reduced ovarian germline stem cell (GSC) proliferation. Female GSCs display high levels of ribosomal RNA (rRNA) transcription, and Udd becomes enriched in GSCs relative to their differentiating daughters. Increasing Pol I transcription delays differentiation, whereas reducing rRNA production induces both morphological changes that accompany multicellular cyst formation and specific decreased expression of the bone morphogenetic protein (BMP) pathway component Mad. These findings demonstrate that modulating rRNA synthesis fosters changes in the cell fate, growth, and proliferation of female Drosophila GSCs and their daughters.

Original languageEnglish (US)
Pages (from-to)298-301
Number of pages4
JournalScience
Volume343
Issue number6168
DOIs
StatePublished - 2014

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Ribosomal RNA
Cell Lineage
Drosophila
Stem Cells
Cell Proliferation
Bone Morphogenetic Proteins
Growth
Ribosomes
Cysts
Oogonial Stem Cells

ASJC Scopus subject areas

  • General

Cite this

Changes in rRNA transcription influence proliferation and cell fate within a stem cell lineage. / Zhang, Qiao; Shalaby, Nevine A.; Buszczak, Michael.

In: Science, Vol. 343, No. 6168, 2014, p. 298-301.

Research output: Contribution to journalArticle

Zhang, Qiao ; Shalaby, Nevine A. ; Buszczak, Michael. / Changes in rRNA transcription influence proliferation and cell fate within a stem cell lineage. In: Science. 2014 ; Vol. 343, No. 6168. pp. 298-301.
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