Changes in the Ir gene controlled response phenotypes in mice of the A(p) and A(k) family of alleles expressing naturally occurring variant molecules

D. M. Shaut, E. K. Wakeland, P. H. Maurer, A. B. Peck

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Several wild-derived H-2 haplotype mice were recently shown by serology and tryptic peptide finger-printing to express I-region A molecules closely related to the A(p) and A(k) molecules of the laboratory strains. To determine if such naturally occurring minor structural variations in the A molecule alter Ir gene-controlled responsiveness, we examined the immune responses of these strains after primary immunizations to three synthetic polypeptide antigens: G60Phe40, GLPhe9, and GAT10. Inbred strains carrying the A(p) (or A(q)) allele are known to be high responders to G60Phe40 and GLPhe9 but low responders to GAT10. Strains expressing the A(k) allele are classified as low responders to G60Phe40 and GLPhe9, but high responders to GAT10. Of seven strains examined belonging to the A(p) family, one (B10.CAS2) failed to respond to either G60Phe40 or GLPhe9 as measured by antibody production and T cell proliferation. In addition, two strains (B10.STC90 and W12A) of the A(k) family were found to be of responder phenotype to GLPhe9. Both GLPhe9 responses resulted from the introduction of new E(β) genes into the I region through naturally occurring intergenic recombination between A(β)A(α) and E(β). All strains of mice in the A(k) family proved to be of the high responder phenotype in their responses toward GAT10. These results contrast strongly with known patterns of alloreactivity against the variant A(p) and A(k) molecules.

Original languageEnglish (US)
Pages (from-to)1410-1416
Number of pages7
JournalJournal of Immunology
Volume133
Issue number3
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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