Changes in the protein pattern of murine B cells after mitogenic stimulation

J. Kettman, I. Lefkovits

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The technique of two-dimensional gel electrophoresis has been applied to study the changes in protein patterns occurring in murine B lymphocytes after activation with the synergistic B cell mitogens lipopolysaccharide and dextran sulfate. Whole cell lysates from [35S]methionine-labeled mitogen-activated B cells were analyzed and radiofluorographs of the gels compared. We found that a large number of proteins appear and disappear during the 5-day activation period (74/206 spots of a nonimmunoglobulin region of the gel). The B cell pattern (in the nonimmunoglobulin region) was also compared with that of mitogen-activated T cells. We identified 19 T cell-specific and 29 B cell-specific proteins, the remaining 1000 or so proteins being common.

Original languageEnglish (US)
Pages (from-to)778-781
Number of pages4
JournalEuropean Journal of Immunology
Volume14
Issue number9
StatePublished - 1984

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B-Lymphocytes
Mitogens
Proteins
Gels
T-Lymphocytes
Dextran Sulfate
Electrophoresis, Gel, Two-Dimensional
Lymphocyte Activation
Methionine
Lipopolysaccharides

ASJC Scopus subject areas

  • Immunology

Cite this

Changes in the protein pattern of murine B cells after mitogenic stimulation. / Kettman, J.; Lefkovits, I.

In: European Journal of Immunology, Vol. 14, No. 9, 1984, p. 778-781.

Research output: Contribution to journalArticle

Kettman, J. ; Lefkovits, I. / Changes in the protein pattern of murine B cells after mitogenic stimulation. In: European Journal of Immunology. 1984 ; Vol. 14, No. 9. pp. 778-781.
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