Chapter 24 Hippocampal grafts derived from embryonic trisomy 16 mice exhibit amyloid (A4) and neurofibrillary pathology

S. J. Richards, J. J. Waters, D. C. Rogers, F. L. Martel, D. R. Sparkman, C. L. White, K. Beyreuther, C. L. Masters, S. B. Dunnett

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15 Scopus citations

Abstract

This chapter discusses the transplanted neocortical and hippocampal tissues derived from trisomic and control fetuses into the brains of normal recipient mice and monitored neuropathologcal changes occurring over 4–6 months within the grafts. In the experiments discussed in the chapter, embryos for transplantation were staged by vaginal plugs and the developmental age was confirmed by crown-rump length. Frontal cortex and hippocampal dissections were taken from 7 trisomic and 18 normal embryos assessed as E14-16. The trisomy 16 embryo was devoid of immunoreactivity to either antiserum. Neocortical and/or hippocampal sections derived from post mortem Alzheimer brain were included in each batch of immunocytochemical staining to confirm the activity and appropriate patterns of immunoreactivity of the antibodies. lmmunocytochemical staining with an anti-A4 antibody demonstrated the presence of deposits of A4 immunoreactivity around the cerebral vasculature within the trisomic grafts. The intracellular immunoreactivity of similar numbers of cells within trisomic grafts was observed with the A128 antiserum raised against purified paired helical filaments.

Original languageEnglish (US)
Pages (from-to)215-223
Number of pages9
JournalProgress in Brain Research
Volume82
Issue numberC
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Neuroscience(all)

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