Characterisation of an outer membrane protein of Moraxella catarrhalis

Kate E. Mathers, David Goldblatt, Christoph Aebi, Rong Hua Yu, Anthony B. Schryvers, Eric J. Hansen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To elucidate potential vaccine antigens, Moraxella catarrhalis outer membrane proteins (OMPs) were studied. We have previously shown an OMP to be a target for human IgG and have now further characterised this OMP which appears to have a molecular mass of 84 kDa and to be distinct from the 81-kDa OMP, CopB. Human transferrin was shown to bind the 84-kDa Chip alone. N-terminal sequencing of this OMP and purified M. catarrhalis transferrin binding protein B (TbpB) revealed homolog both with each other and with the TbpB of Haemophilus influenzae and Neisseria meningitidis. Adsorption of human anti-serum with purified TbpB from two M. catarrhalis strains abolished or reduced binding of IgG to the 84-kDa OMP from thee M. catarrhalis isolates. IgG binding to CopB was unaffected. It is clear that the 84-kDa OMP is distinct from CopB and is a likely homologue of TbpB.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalFEMS Immunology and Medical Microbiology
Volume19
Issue number3
DOIs
StatePublished - Nov 1997

Fingerprint

Moraxella (Branhamella) catarrhalis
Transferrin-Binding Protein B
Membrane Proteins
Immunoglobulin G
Neisseria meningitidis
Haemophilus influenzae
Transferrin
Adsorption
Vaccines
Antigens
Serum

Keywords

  • Immunoglobulin G
  • Moraxella catarrhalis
  • Outer membrane protein
  • Transferrin binding protein

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

Mathers, K. E., Goldblatt, D., Aebi, C., Yu, R. H., Schryvers, A. B., & Hansen, E. J. (1997). Characterisation of an outer membrane protein of Moraxella catarrhalis. FEMS Immunology and Medical Microbiology, 19(3), 231-236. https://doi.org/10.1016/S0928-8244(97)00088-6

Characterisation of an outer membrane protein of Moraxella catarrhalis. / Mathers, Kate E.; Goldblatt, David; Aebi, Christoph; Yu, Rong Hua; Schryvers, Anthony B.; Hansen, Eric J.

In: FEMS Immunology and Medical Microbiology, Vol. 19, No. 3, 11.1997, p. 231-236.

Research output: Contribution to journalArticle

Mathers, KE, Goldblatt, D, Aebi, C, Yu, RH, Schryvers, AB & Hansen, EJ 1997, 'Characterisation of an outer membrane protein of Moraxella catarrhalis', FEMS Immunology and Medical Microbiology, vol. 19, no. 3, pp. 231-236. https://doi.org/10.1016/S0928-8244(97)00088-6
Mathers, Kate E. ; Goldblatt, David ; Aebi, Christoph ; Yu, Rong Hua ; Schryvers, Anthony B. ; Hansen, Eric J. / Characterisation of an outer membrane protein of Moraxella catarrhalis. In: FEMS Immunology and Medical Microbiology. 1997 ; Vol. 19, No. 3. pp. 231-236.
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