Characteristics and autoradiographic localization of 2-[125I]iodomelatonin binding sites in djungarian hamster brain

M. J. Duncan, J. S. Takahashi, M. L. Dubocovich

Research output: Contribution to journalArticle

134 Scopus citations

Abstract

These studies investigated the characteristics and regional distribution of 2-[125I]iodomelatonin binding in Djungarian hamster brain. The results showed that 2-[125I]iodomelatonin labels two types of binding sites, which resemble the ML-1 and ML-2 melatonin subtypes previously described in other tissues. The 2-[125I]iodomelatonin binding site identified in whole brain membranes has a nanomolar affinity (Kd = 1.48 ± 0.26 nM) and biochemical and pharmacological characteristics identical to those of the ML-2 site of Syrian hamster whole brain. The 2-[125I]iodomelatonin site in the hypothalamus has a picomolar affinity (Kd = 43.4 ± 5.1 pM) and resembles the ML-1 site of chicken retina. The localization of 2-[125I]iodomelatonin labeling in autoradiographic studies of the Djungarian hamster brain includes the suprachiasmatic nuclei, the median eminence, the reuniens nucleus, and the paraventricular nucleus of the thalamus.

Original languageEnglish (US)
Pages (from-to)1011-1018
Number of pages8
JournalEndocrinology
Volume125
Issue number2
DOIs
StatePublished - Aug 1989

ASJC Scopus subject areas

  • Endocrinology

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