Characteristics of cell lines established from human colorectal carcinoma

J. G. Park, H. K. Oie, P. H. Sugarbaker, J. G. Henslee, T. R. Chen, B. E. Johnson, A. Gazdar

Research output: Contribution to journalArticle

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Abstract

We have characterized 14 human colorectal carcinoma cell lines established from primary and metastatic sites by us during the years 1982 to 1985. Five lines were established in fully defined ACL-4 medium and 9 in serum supplemented R10 medium. However, after establishment, cultures could be grown interchangeably in either medium. The lines grew as floating cell aggregates in ACL-4 medium, while most demonstrated substrate adherence in R10 medium. The lines had relatively long doubling times and low cloning efficiencies. Twelve were tumorigenic in athymic nude mice when injected s.c., and two grew i.p. as well. Based on culture, xenograft, and ultrastructural morpholoiges, the 14 lines could be subtyped as follows: 4 were well differentiated; 5 were moderately differentiated; 4 were poorly differentiated; and 1 was a mucinous carcinoma. Membrane associated antigens characteristic for gastrointestinal cells (carcinoembryonic antigen, CA 19-9, and TAG-72 antigens) were expressed by 50-71% of the lines. Lines expressing carcinoembryonic antigen and CA 19-9 actively secreted these antigens into the supernatant fluids while TAG-72 antigen was not secreted. Surprisingly, 5 of 7 of the original tumor samples tested and 13 of 14 cultured lines expressed L-dopa decarboxylase activity, which is a characteristic enzyme marker of neuroendocrine cells and tumors. In addition, one poorly differentiated cell line contained dense core granules, characteristic of endocrine secretion. Preliminary cytogenetic analyses indicated that 9 of 11 lines examined contained double minute chromosomes. In addition, 3 of the 9 lines with double minutes also had homogeneously staining regions. These findings indicate a high incidence of amplification of one or more as yet unidentified genes.

Original languageEnglish (US)
Pages (from-to)6710-6718
Number of pages9
JournalCancer Research
Volume47
Issue number24 I
StatePublished - 1987

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Colorectal Neoplasms
Antigens
Cell Line
Carcinoembryonic Antigen
Nude Mice
Dopa Decarboxylase
Mucinous Adenocarcinoma
Neuroendocrine Cells
Neuroendocrine Tumors
Cytogenetic Analysis
Levodopa
Heterografts
Organism Cloning
Chromosomes
Staining and Labeling
Membranes
Incidence
Enzymes
Serum
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Park, J. G., Oie, H. K., Sugarbaker, P. H., Henslee, J. G., Chen, T. R., Johnson, B. E., & Gazdar, A. (1987). Characteristics of cell lines established from human colorectal carcinoma. Cancer Research, 47(24 I), 6710-6718.

Characteristics of cell lines established from human colorectal carcinoma. / Park, J. G.; Oie, H. K.; Sugarbaker, P. H.; Henslee, J. G.; Chen, T. R.; Johnson, B. E.; Gazdar, A.

In: Cancer Research, Vol. 47, No. 24 I, 1987, p. 6710-6718.

Research output: Contribution to journalArticle

Park, JG, Oie, HK, Sugarbaker, PH, Henslee, JG, Chen, TR, Johnson, BE & Gazdar, A 1987, 'Characteristics of cell lines established from human colorectal carcinoma', Cancer Research, vol. 47, no. 24 I, pp. 6710-6718.
Park JG, Oie HK, Sugarbaker PH, Henslee JG, Chen TR, Johnson BE et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Research. 1987;47(24 I):6710-6718.
Park, J. G. ; Oie, H. K. ; Sugarbaker, P. H. ; Henslee, J. G. ; Chen, T. R. ; Johnson, B. E. ; Gazdar, A. / Characteristics of cell lines established from human colorectal carcinoma. In: Cancer Research. 1987 ; Vol. 47, No. 24 I. pp. 6710-6718.
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