Characteristics of DNA-binding proteins determine the biological sensitivity to high-linear energy transfer radiation

Hongyan Wang, Xiangming Zhang, Ping Wang, Xiaoyan Yu, Jeroen Essers, David Chen, Roland Kanaar, Shunichi Takeda, Ya Wang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Non-homologous end-joining (NHEJ) and homologous recombination repair (HRR), contribute to repair ionizing radiation (IR)-induced DNA double-strand breaks (DSBs). Mre11 binding to DNA is the first step for activating HRR and Ku binding to DNA is the first step for initiating NHEJ. High-linear energy transfer (LET) IR (such as high energy charged particles) killing more cells at the same dose as compared with low-LET IR (such as X or γ rays) is due to inefficient NHEJ. However, these phenomena have not been demonstrated at the animal level and the mechanism by which high-LET IR does not affect the efficiency of HRR remains unclear. In this study, we showed that although wild-type and HRR-deficient mice or DT40 cells are more sensitive to high-LET IR than to low-LET IR, NHEJ deficient mice or DT40 cells are equally sensitive to high-and low-LET IR. We also showed that Mre11 and Ku respond differently to shorter DNA fragments in vitro and to the DNA from high-LET irradiated cells in vivo. These findings provide strong evidence that the different DNA DSB binding properties of Mre11 and Ku determine the different efficiencies of HRR and NHEJ to repair high-LET radiation induced DSBs.

Original languageEnglish (US)
Article numbergkq069
Pages (from-to)3245-3251
Number of pages7
JournalNucleic Acids Research
Volume38
Issue number10
DOIs
StatePublished - Feb 11 2010

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Linear Energy Transfer
DNA-Binding Proteins
Recombinational DNA Repair
Ionizing Radiation
Radiation
Double-Stranded DNA Breaks
DNA
Nonionizing Radiation
X-Rays

ASJC Scopus subject areas

  • Genetics

Cite this

Characteristics of DNA-binding proteins determine the biological sensitivity to high-linear energy transfer radiation. / Wang, Hongyan; Zhang, Xiangming; Wang, Ping; Yu, Xiaoyan; Essers, Jeroen; Chen, David; Kanaar, Roland; Takeda, Shunichi; Wang, Ya.

In: Nucleic Acids Research, Vol. 38, No. 10, gkq069, 11.02.2010, p. 3245-3251.

Research output: Contribution to journalArticle

Wang, H, Zhang, X, Wang, P, Yu, X, Essers, J, Chen, D, Kanaar, R, Takeda, S & Wang, Y 2010, 'Characteristics of DNA-binding proteins determine the biological sensitivity to high-linear energy transfer radiation', Nucleic Acids Research, vol. 38, no. 10, gkq069, pp. 3245-3251. https://doi.org/10.1093/nar/gkq069
Wang, Hongyan ; Zhang, Xiangming ; Wang, Ping ; Yu, Xiaoyan ; Essers, Jeroen ; Chen, David ; Kanaar, Roland ; Takeda, Shunichi ; Wang, Ya. / Characteristics of DNA-binding proteins determine the biological sensitivity to high-linear energy transfer radiation. In: Nucleic Acids Research. 2010 ; Vol. 38, No. 10. pp. 3245-3251.
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