Characteristics of fatty acid oxidation in rat liver homogenates and the inhibitory effect of malonyl-CoA

J. Denis McGarry, Guy P. Mannaerts, Daniel W. Foster

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Experiments were carried out to study the control of fatty acid oxidation and ketogenesis in rat liver homogenates. In contrast to findings with the perfused liver, rates of fatty acid oxidation were high and equal in liver homogenates from fed and fasted animals. No difference in apparent Km values for oleate, ATP, coenzyme A or carnitine could be detected in the two types of homogenate. Over the concentration range 25-40 μM, malonyl-CoA inhibited oleate oxidation by 50-75%. The fact that the inhibitory effect could be removed by pre-treatment with alkali or fatty acid synthetase indicated that the inhibitory molecule was malonyl-CoA rather than a contaminant. The effect was readily reversible and appeared to be competitive with oleyl-CoA. Malonyl-CoA also inhibited oleate oxidation in homogenates of heart and kidney cortex but this is unlikely to have physiological relevance since, in contrast to liver, neither tissue contains an active cytosolic pathway for the generation of malonyl-CoA and the synthesis of fatty acids.

Original languageEnglish (US)
Pages (from-to)305-313
Number of pages9
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume530
Issue number3
DOIs
StatePublished - Sep 28 1978

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Malonyl Coenzyme A
Liver
Rats
Fatty Acids
Oleic Acid
Oxidation
Kidney Cortex
Fatty Acid Synthases
Carnitine
Alkalies
Coenzyme A
Animals
Adenosine Triphosphate
Impurities
Tissue
Molecules
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology
  • Medicine(all)

Cite this

Characteristics of fatty acid oxidation in rat liver homogenates and the inhibitory effect of malonyl-CoA. / McGarry, J. Denis; Mannaerts, Guy P.; Foster, Daniel W.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 530, No. 3, 28.09.1978, p. 305-313.

Research output: Contribution to journalArticle

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