Characterization and expression of a cDNA encoding the human androgen receptor

W. D. Tilley, M. Marcelli, J. D. Wilson, M. J. McPhaul

Research output: Contribution to journalArticle

383 Citations (Scopus)

Abstract

We report the isolation of a cDNA that encodes the complete human androgen receptor. The cDNA predicts a protein of 917 amino acids with a molecular weight of 98,918. Introduction of the cDNA into heterologous mammalian cells caused expression of high levels of a protein that binds dihydrotestosterone with the affinity, specificity, and sedimentation properties characteristic of the native human androgen receptor. Comparisons with the amino acid sequence of previously cloned steroid hormone receptors revealed a high degree of sequence conservation with the progesterone, glucocorticoid, and mineralocorticoid receptors in the putative hormone and DNA-binding domain regions.

Original languageEnglish (US)
Pages (from-to)327-331
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number1
StatePublished - 1989

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Complementary DNA
Hormones
Mineralocorticoid Receptors
Dihydrotestosterone
Steroid Receptors
Glucocorticoid Receptors
Progesterone Receptors
Population Groups
Amino Acid Sequence
Proteins
Molecular Weight
Amino Acids
DNA
human AR protein

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Characterization and expression of a cDNA encoding the human androgen receptor. / Tilley, W. D.; Marcelli, M.; Wilson, J. D.; McPhaul, M. J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 1, 1989, p. 327-331.

Research output: Contribution to journalArticle

Tilley, W. D. ; Marcelli, M. ; Wilson, J. D. ; McPhaul, M. J. / Characterization and expression of a cDNA encoding the human androgen receptor. In: Proceedings of the National Academy of Sciences of the United States of America. 1989 ; Vol. 86, No. 1. pp. 327-331.
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