Characterization and expression of a cDNA encoding the human androgen receptor

W. D. Tilley, M. Marcelli, J. D. Wilson, M. J. McPhaul

Research output: Contribution to journalArticlepeer-review

388 Scopus citations

Abstract

We report the isolation of a cDNA that encodes the complete human androgen receptor. The cDNA predicts a protein of 917 amino acids with a molecular weight of 98,918. Introduction of the cDNA into heterologous mammalian cells caused expression of high levels of a protein that binds dihydrotestosterone with the affinity, specificity, and sedimentation properties characteristic of the native human androgen receptor. Comparisons with the amino acid sequence of previously cloned steroid hormone receptors revealed a high degree of sequence conservation with the progesterone, glucocorticoid, and mineralocorticoid receptors in the putative hormone and DNA-binding domain regions.

Original languageEnglish (US)
Pages (from-to)327-331
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number1
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • General

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