Characterization of β-adrenergic receptors in cultured human trabecular cells and in human trabecular meshwork

M. B. Wax, P. B. Molinoff, J. Alvarado, J. Polansky

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The characterization of β-adrenergic receptors on cultured human trabecular cells and trabecular meshwork from human autopsy eyes was carried out by radioligand bindinb utilizing (125I)-iodopindolol. In cultured cells, the observed binding of (125I)-iodopindolol was of high affinity (K(d) = 43 pM) and saturable. Scatchard plots were linear and revealed a B(max) of 33 ± 7 fmol/mg of protein. Competition studies with a series of agonists and antagonists revealed that human trabecular cells contain a single class of β-adrenergic receptors of the β2 subtype. Similarly, the IC50 of ICI 89,406 (176 nM) in human trabecular meshwork from autopsy eyes supports the presence of β2-adrenergic receptors in this tissue.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume30
Issue number1
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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