Abstract
The characterization of β-adrenergic receptors on cultured human trabecular cells and trabecular meshwork from human autopsy eyes was carried out by radioligand bindinb utilizing (125I)-iodopindolol. In cultured cells, the observed binding of (125I)-iodopindolol was of high affinity (K(d) = 43 pM) and saturable. Scatchard plots were linear and revealed a B(max) of 33 ± 7 fmol/mg of protein. Competition studies with a series of agonists and antagonists revealed that human trabecular cells contain a single class of β-adrenergic receptors of the β2 subtype. Similarly, the IC50 of ICI 89,406 (176 nM) in human trabecular meshwork from autopsy eyes supports the presence of β2-adrenergic receptors in this tissue.
Original language | English (US) |
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Pages (from-to) | 51-57 |
Number of pages | 7 |
Journal | Investigative Ophthalmology and Visual Science |
Volume | 30 |
Issue number | 1 |
State | Published - 1989 |
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience