The characterization of β-adrenergic receptors on cultured human trabecular cells and trabecular meshwork from human autopsy eyes was carried out by radioligand bindinb utilizing (125I)-iodopindolol. In cultured cells, the observed binding of (125I)-iodopindolol was of high affinity (K(d) = 43 pM) and saturable. Scatchard plots were linear and revealed a B(max) of 33 ± 7 fmol/mg of protein. Competition studies with a series of agonists and antagonists revealed that human trabecular cells contain a single class of β-adrenergic receptors of the β2 subtype. Similarly, the IC50 of ICI 89,406 (176 nM) in human trabecular meshwork from autopsy eyes supports the presence of β2-adrenergic receptors in this tissue.
|Original language||English (US)|
|Number of pages||7|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - 1989|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience