Characterization of 13 novel microsatellite markers in the Galápagos tortoise (Chelonoidis nigra)

Sébastien Rioux Paquette; Shannon E. Engberg; Ryan M. Huebinger; Edward E. Louis

doi:10.1007/s12686-012-9640-7

Characterization of 13 novel microsatellite markers in the Galápagos tortoise (Chelonoidis nigra)

Sébastien Rioux Paquette, Shannon E. Engberg, Ryan M. Huebinger, Edward E. Louis

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Galápagos giant tortoises (Chelonoidis nigra ssp.) are widely known for both the role played in evolutionary theory and their precarious conservation status. Thirteen nuclear microsatellite loci were isolated and tested on 60 individuals from Cinco Cerros, Isabela Island. The mean number of alleles per locus was 5.5 (range between 2 and 12) while mean observed and expected heterozygosity were 0.417 and 0.426, respectively. A single marker statistically deviated from Hardy-Weinberg equilibrium. These markers represent a valuable addition to the molecular tools available for studying the taxonomic affiliation and relatedness of individuals in captive management plans, crucial for the maintenance of several subspecies.

Original language	English (US)
Pages (from-to)	773-776
Number of pages	4
Journal	Conservation Genetics Resources
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/s12686-012-9640-7
State	Published - Sep 2012

Keywords

Chelonoidis nigra
Galápagos Islands
Galápagos tortoise
Genetic markers
Microsatellites

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-012-9640-7

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title = "Characterization of 13 novel microsatellite markers in the Gal{\'a}pagos tortoise (Chelonoidis nigra)",

abstract = "Gal{\'a}pagos giant tortoises (Chelonoidis nigra ssp.) are widely known for both the role played in evolutionary theory and their precarious conservation status. Thirteen nuclear microsatellite loci were isolated and tested on 60 individuals from Cinco Cerros, Isabela Island. The mean number of alleles per locus was 5.5 (range between 2 and 12) while mean observed and expected heterozygosity were 0.417 and 0.426, respectively. A single marker statistically deviated from Hardy-Weinberg equilibrium. These markers represent a valuable addition to the molecular tools available for studying the taxonomic affiliation and relatedness of individuals in captive management plans, crucial for the maintenance of several subspecies.",

keywords = "Chelonoidis nigra, Gal{\'a}pagos Islands, Gal{\'a}pagos tortoise, Genetic markers, Microsatellites",

author = "Paquette, {S{\'e}bastien Rioux} and Engberg, {Shannon E.} and Huebinger, {Ryan M.} and Louis, {Edward E.}",

note = "Funding Information: Acknowledgments The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. We would also like to acknowledge Philadelphia Zoo for their initial support for the development of this marker suite. We graciously thank the Theodore F. and Claire M. Hubbard Family Foundation and the Ahmanson Foundation for their support of Omaha{\textquoteright}s Henry Doorly Zoo{\textquoteright}s Center for Conservation and Research. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2012",

month = sep,

doi = "10.1007/s12686-012-9640-7",

language = "English (US)",

volume = "4",

pages = "773--776",

journal = "Conservation Genetics Resources",

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publisher = "Springer Verlag",

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AU - Paquette, Sébastien Rioux

AU - Engberg, Shannon E.

AU - Huebinger, Ryan M.

AU - Louis, Edward E.

N1 - Funding Information: Acknowledgments The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. We would also like to acknowledge Philadelphia Zoo for their initial support for the development of this marker suite. We graciously thank the Theodore F. and Claire M. Hubbard Family Foundation and the Ahmanson Foundation for their support of Omaha’s Henry Doorly Zoo’s Center for Conservation and Research. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2012/9

Y1 - 2012/9

N2 - Galápagos giant tortoises (Chelonoidis nigra ssp.) are widely known for both the role played in evolutionary theory and their precarious conservation status. Thirteen nuclear microsatellite loci were isolated and tested on 60 individuals from Cinco Cerros, Isabela Island. The mean number of alleles per locus was 5.5 (range between 2 and 12) while mean observed and expected heterozygosity were 0.417 and 0.426, respectively. A single marker statistically deviated from Hardy-Weinberg equilibrium. These markers represent a valuable addition to the molecular tools available for studying the taxonomic affiliation and relatedness of individuals in captive management plans, crucial for the maintenance of several subspecies.

AB - Galápagos giant tortoises (Chelonoidis nigra ssp.) are widely known for both the role played in evolutionary theory and their precarious conservation status. Thirteen nuclear microsatellite loci were isolated and tested on 60 individuals from Cinco Cerros, Isabela Island. The mean number of alleles per locus was 5.5 (range between 2 and 12) while mean observed and expected heterozygosity were 0.417 and 0.426, respectively. A single marker statistically deviated from Hardy-Weinberg equilibrium. These markers represent a valuable addition to the molecular tools available for studying the taxonomic affiliation and relatedness of individuals in captive management plans, crucial for the maintenance of several subspecies.

KW - Chelonoidis nigra

KW - Galápagos Islands

KW - Galápagos tortoise

KW - Genetic markers

KW - Microsatellites

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Characterization of 13 novel microsatellite markers in the Galápagos tortoise (Chelonoidis nigra)

Abstract

Keywords

ASJC Scopus subject areas

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