Characterization of 13 novel microsatellite markers in the Galápagos tortoise (Chelonoidis nigra)

Sébastien Rioux Paquette, Shannon E. Engberg, Ryan M. Huebinger, Edward E. Louis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Galápagos giant tortoises (Chelonoidis nigra ssp.) are widely known for both the role played in evolutionary theory and their precarious conservation status. Thirteen nuclear microsatellite loci were isolated and tested on 60 individuals from Cinco Cerros, Isabela Island. The mean number of alleles per locus was 5.5 (range between 2 and 12) while mean observed and expected heterozygosity were 0.417 and 0.426, respectively. A single marker statistically deviated from Hardy-Weinberg equilibrium. These markers represent a valuable addition to the molecular tools available for studying the taxonomic affiliation and relatedness of individuals in captive management plans, crucial for the maintenance of several subspecies.

Original languageEnglish (US)
Pages (from-to)773-776
Number of pages4
JournalConservation Genetics Resources
Volume4
Issue number3
DOIs
StatePublished - 2012

Fingerprint

Turtles
tortoises
Islands
Microsatellite Repeats
Alleles
Maintenance
microsatellite repeats
loci
evolutionary theory
conservation status
relatedness
heterozygosity
subspecies
allele
alleles
Chelonoidis nigra
marker
tortoise
management plan

Keywords

  • Chelonoidis nigra
  • Galápagos Islands
  • Galápagos tortoise
  • Genetic markers
  • Microsatellites

ASJC Scopus subject areas

  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Characterization of 13 novel microsatellite markers in the Galápagos tortoise (Chelonoidis nigra). / Paquette, Sébastien Rioux; Engberg, Shannon E.; Huebinger, Ryan M.; Louis, Edward E.

In: Conservation Genetics Resources, Vol. 4, No. 3, 2012, p. 773-776.

Research output: Contribution to journalArticle

Paquette, Sébastien Rioux ; Engberg, Shannon E. ; Huebinger, Ryan M. ; Louis, Edward E. / Characterization of 13 novel microsatellite markers in the Galápagos tortoise (Chelonoidis nigra). In: Conservation Genetics Resources. 2012 ; Vol. 4, No. 3. pp. 773-776.
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