Characterization of a 3.5-kbp plasmid from Helicobacter pylori

Jennifer A. Minnis, Tammy E. Taylor, John E. Knesek, Walter L. Peterson, Sarah A. McIntire

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A 3.5-kbp plasmid (pHPM180) was isolated from Helicobacter pylori (HPM180) and the DNA sequence was determined. Two open reading frames (ORF1 and ORF2) were identified which could encode polypeptides of 54,517 and 27,629 Da, respectively. Ribosome binding and promoter consensus sequences were identified, as well as two 232-bp direct repeats and four 22 bp direct repeats. DNA sequence homology was found between pHPM180 ORF1 and a 684-base pair HindIII fragment from a 5.6 kbp H. pylori plasmid. ORF1 showed amino acid homology with six replication proteins from bacterial plasmids with theta-type replicons. Additional sequence identity was found between pHPM 180 noncoding DNA and a segment of H. pylori pHPK255, a plasmid that replicates via a rolling circle type mechanism. A ribonuclease protection assay determined that ORF1 was transcribed in H. pylori HPM180.

Original languageEnglish (US)
Pages (from-to)22-36
Number of pages15
JournalPlasmid
Volume34
Issue number1
DOIs
StatePublished - Jul 1995

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint Dive into the research topics of 'Characterization of a 3.5-kbp plasmid from Helicobacter pylori'. Together they form a unique fingerprint.

  • Cite this

    Minnis, J. A., Taylor, T. E., Knesek, J. E., Peterson, W. L., & McIntire, S. A. (1995). Characterization of a 3.5-kbp plasmid from Helicobacter pylori. Plasmid, 34(1), 22-36. https://doi.org/10.1006/plas.1995.1030