Characterization of a 3.5-kbp Plasmid from Helicobacter pylori

Jennifer A. Minnis, Tammy E. Taylor, John E. Knesek, Walter L. Peterson, Sarah A. McIntire

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A 3.5-kbp plasmid (pHPM180) was isolated from Helicobacter pylori (HPM180) and the DNA sequence was determined. Two open reading frames (ORF1 and ORF2) were identified which could encode polypeptides of 54,517 and 27,629 Da, respectively. Ribosome binding and promoter consensus sequences were identified, as well as two 232-bp direct repeats and four 22-bp direct repeats. DNA sequence homology was found between pHPM180 ORF1 and a 684-base pair Hin dIII fragment from a 5.6 kbp H. pylori plasmid. ORF1 showed amino acid homology with six replication proteins from bacterial plasmids with theta-type replicons. Additional sequence identity was found between pHPM180 noncoding DNA and a segment of H. pylori pHPK255, a plasmid that replicates via a rolling circle type mechanism. A ribonuclease protection assay determined that ORF1 was transcribed in H. pylori HPM180.

Original languageEnglish (US)
Pages (from-to)22-36
Number of pages15
JournalPlasmid
Volume34
Issue number1
DOIs
StatePublished - Jul 1995

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Helicobacter pylori
Plasmids
Nucleic Acid Repetitive Sequences
Replicon
Bacterial Proteins
Consensus Sequence
Ribonucleases
Sequence Homology
Ribosomes
Base Pairing
Open Reading Frames
Amino Acids
Peptides
DNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Minnis, J. A., Taylor, T. E., Knesek, J. E., Peterson, W. L., & McIntire, S. A. (1995). Characterization of a 3.5-kbp Plasmid from Helicobacter pylori. Plasmid, 34(1), 22-36. https://doi.org/10.1006/plas.1995.1030

Characterization of a 3.5-kbp Plasmid from Helicobacter pylori. / Minnis, Jennifer A.; Taylor, Tammy E.; Knesek, John E.; Peterson, Walter L.; McIntire, Sarah A.

In: Plasmid, Vol. 34, No. 1, 07.1995, p. 22-36.

Research output: Contribution to journalArticle

Minnis, JA, Taylor, TE, Knesek, JE, Peterson, WL & McIntire, SA 1995, 'Characterization of a 3.5-kbp Plasmid from Helicobacter pylori', Plasmid, vol. 34, no. 1, pp. 22-36. https://doi.org/10.1006/plas.1995.1030
Minnis JA, Taylor TE, Knesek JE, Peterson WL, McIntire SA. Characterization of a 3.5-kbp Plasmid from Helicobacter pylori. Plasmid. 1995 Jul;34(1):22-36. https://doi.org/10.1006/plas.1995.1030
Minnis, Jennifer A. ; Taylor, Tammy E. ; Knesek, John E. ; Peterson, Walter L. ; McIntire, Sarah A. / Characterization of a 3.5-kbp Plasmid from Helicobacter pylori. In: Plasmid. 1995 ; Vol. 34, No. 1. pp. 22-36.
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