Characterization of a B cell defect in the NZB mouse manifested by an increased ratio of surface IgM to IgD

P. Cohen, M. Ziff, E. S. Vitetta

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Abstract

In an effort to define the cellular basis of abnormalities in polyclonal B cell activation previously noted in NZB mice, the surface immunoglobulin (sIg) isotypes of spleen cells from NZB mice were examined. After lactoperoxidase-catalyzed radioiodination, the cell surface immunoglobulins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Spleen cells from 8- to 10-week-old NZB mice were found to have an increased ratio of cell surface IgM/IgD compared to cells from 11 control strains. The altered ratio of sIg isotypes was not a consequence of increased proteolytic activity present in NZB cell suspensions or of the presence of cytophilic antibody or autoantibody. Ontogenetic studies of the sIgM/sIgD (μ/δ) ratio on splenocytes from NZB and BALB/c mice revealed that the former cells had higher μ/δ ratios as early as 2 weeks after birth. By 4 weeks of age the μ/δ ratios were equivalent. Between 4 weeks and 1 year of age, the μ/δ ratios on NZB splenocytes remained constant whereas those on BALB/c splenocytes decreased and reached adult levels at 6 weeks.

Original languageEnglish (US)
Pages (from-to)973-977
Number of pages5
JournalJournal of Immunology
Volume121
Issue number3
StatePublished - 1978

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Inbred NZB Mouse
Immunoglobulin D
Immunoglobulin M
B-Lymphocytes
B-Cell Antigen Receptors
Immunoglobulin Isotypes
Spleen
Lactoperoxidase
Sodium Dodecyl Sulfate
Autoantibodies
Polyacrylamide Gel Electrophoresis
Suspensions
Parturition
Antibodies

ASJC Scopus subject areas

  • Immunology

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Characterization of a B cell defect in the NZB mouse manifested by an increased ratio of surface IgM to IgD. / Cohen, P.; Ziff, M.; Vitetta, E. S.

In: Journal of Immunology, Vol. 121, No. 3, 1978, p. 973-977.

Research output: Contribution to journalArticle

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