Characterization of a cdc14 null allele in Drosophila melanogaster

Leif R. Neitzel, Matthew R. Broadus, Nailing Zhang, Leah Sawyer, Heather A. Wallace, Julie A. Merkle, Jeanne N. Jodoin, Poojitha Sitaram, Emily E. Crispi, William Rork, Laura A. Lee, Duojia Pan Ph.D., Kathleen L. Gould, Andrea Page-McCaw, Ethan Lee

Research output: Contribution to journalArticle

Abstract

Cdc14 is an evolutionarily conserved serine/threonine phosphatase. Originally identified in Saccharomyces cerevisiae as a cell cycle regulator, its role in other eukaryotic organisms remains unclear. In Drosophila melanogaster, Cdc14 is encoded by a single gene, thus facilitating its study. We found that Cdc14 expression is highest in the testis of adult flies and that cdc14 null flies are viable. cdc14 null female and male flies do not display altered fertility. cdc14 null males, however, exhibit decreased sperm competitiveness. Previous studies have shown that Cdc14 plays a role in ciliogenesis during zebrafish development. In Drosophila, sensory neurons are ciliated. We found that the Drosophila cdc14 null mutants have defects in chemosensation and mechanosensation as indicated by decreased avoidance of repellant substances and decreased response to touch. In addition, we show that cdc14 null mutants have defects in lipid metabolism and resistance to starvation. These studies highlight the diversity of Cdc14 function in eukaryotes despite its structural conservation.

Original languageEnglish (US)
Article numberbio035394
JournalBiology Open
Volume7
Issue number7
DOIs
StatePublished - Jan 1 2018

Fingerprint

null alleles
Drosophila melanogaster
Diptera
Alleles
Defects
Drosophila
Phosphoprotein Phosphatases
Yeast
Neurons
Conservation
mutants
Genes
sensory neurons
Cells
touch (sensation)
Touch
Zebrafish
Sensory Receptor Cells
Starvation
Eukaryota

Keywords

  • Cdc14
  • Chemosensation
  • Drosophila
  • Mechanosensation
  • Sensilla
  • Sperm

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Neitzel, L. R., Broadus, M. R., Zhang, N., Sawyer, L., Wallace, H. A., Merkle, J. A., ... Lee, E. (2018). Characterization of a cdc14 null allele in Drosophila melanogaster. Biology Open, 7(7), [bio035394]. https://doi.org/10.1242/bio.035394

Characterization of a cdc14 null allele in Drosophila melanogaster. / Neitzel, Leif R.; Broadus, Matthew R.; Zhang, Nailing; Sawyer, Leah; Wallace, Heather A.; Merkle, Julie A.; Jodoin, Jeanne N.; Sitaram, Poojitha; Crispi, Emily E.; Rork, William; Lee, Laura A.; Pan Ph.D., Duojia; Gould, Kathleen L.; Page-McCaw, Andrea; Lee, Ethan.

In: Biology Open, Vol. 7, No. 7, bio035394, 01.01.2018.

Research output: Contribution to journalArticle

Neitzel, LR, Broadus, MR, Zhang, N, Sawyer, L, Wallace, HA, Merkle, JA, Jodoin, JN, Sitaram, P, Crispi, EE, Rork, W, Lee, LA, Pan Ph.D., D, Gould, KL, Page-McCaw, A & Lee, E 2018, 'Characterization of a cdc14 null allele in Drosophila melanogaster', Biology Open, vol. 7, no. 7, bio035394. https://doi.org/10.1242/bio.035394
Neitzel LR, Broadus MR, Zhang N, Sawyer L, Wallace HA, Merkle JA et al. Characterization of a cdc14 null allele in Drosophila melanogaster. Biology Open. 2018 Jan 1;7(7). bio035394. https://doi.org/10.1242/bio.035394
Neitzel, Leif R. ; Broadus, Matthew R. ; Zhang, Nailing ; Sawyer, Leah ; Wallace, Heather A. ; Merkle, Julie A. ; Jodoin, Jeanne N. ; Sitaram, Poojitha ; Crispi, Emily E. ; Rork, William ; Lee, Laura A. ; Pan Ph.D., Duojia ; Gould, Kathleen L. ; Page-McCaw, Andrea ; Lee, Ethan. / Characterization of a cdc14 null allele in Drosophila melanogaster. In: Biology Open. 2018 ; Vol. 7, No. 7.
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