Characterization of a novel ghrelin cell reporter mouse

Ichiro Sakata, Yoshihide Nakano, Sherri Osborne-Lawrence, Sherry A. Rovinsky, Charlotte E. Lee, Mario Perello, Jason G. Anderson, Roberto Coppari, Guanghua Xiao, Bradford B. Lowell, Joel K. Elmquist, Jeffrey M. Zigman

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Ghrelin is a hormone that influences many physiological processes and behaviors, such as food intake, insulin and growth hormone release, and a coordinated response to chronic stress. However, little is known about the molecular pathways governing ghrelin release and ghrelin cell function. To better study ghrelin cell physiology, we have generated several transgenic mouse lines expressing humanized Renilla reniformis green fluorescent protein (hrGFP) under the control of the mouse ghrelin promoter. hrGFP expression was especially abundant in the gastric oxyntic mucosa, in a pattern mirroring that of ghrelin immunoreactivity and ghrelin mRNA. hrGFP expression also was observed in the duodenum, but not in the brain, pancreatic islet, or testis. In addition, we used fluorescent activated cell sorting (FACS) to collect and partially characterize highly enriched populations of gastric ghrelin cells. We suggest that these novel ghrelin-hrGFP transgenic mice will serve as useful tools to better understand ghrelin cell physiology.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalRegulatory Peptides
Volume155
Issue number1-3
DOIs
Publication statusPublished - Jun 5 2009

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Keywords

  • FACS
  • Ghrelin
  • Green fluorescence protein
  • Transgenic

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Sakata, I., Nakano, Y., Osborne-Lawrence, S., Rovinsky, S. A., Lee, C. E., Perello, M., ... Zigman, J. M. (2009). Characterization of a novel ghrelin cell reporter mouse. Regulatory Peptides, 155(1-3), 91-98. https://doi.org/10.1016/j.regpep.2009.04.001