Characterization of a novel ghrelin cell reporter mouse

Ichiro Sakata; Yoshihide Nakano; Sherri Osborne-Lawrence; Sherry A. Rovinsky; Charlotte E. Lee; Mario Perello; Jason G. Anderson; Roberto Coppari; Guanghua Xiao; Bradford B. Lowell; Joel K. Elmquist; Jeffrey M. Zigman

doi:10.1016/j.regpep.2009.04.001

Characterization of a novel ghrelin cell reporter mouse

Ichiro Sakata, Yoshihide Nakano, Sherri Osborne-Lawrence, Sherry A. Rovinsky, Charlotte E. Lee, Mario Perello, Jason G. Anderson, Roberto Coppari, Guanghua Xiao, Bradford B. Lowell, Joel K. Elmquist, Jeffrey M. Zigman

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

Ghrelin is a hormone that influences many physiological processes and behaviors, such as food intake, insulin and growth hormone release, and a coordinated response to chronic stress. However, little is known about the molecular pathways governing ghrelin release and ghrelin cell function. To better study ghrelin cell physiology, we have generated several transgenic mouse lines expressing humanized Renilla reniformis green fluorescent protein (hrGFP) under the control of the mouse ghrelin promoter. hrGFP expression was especially abundant in the gastric oxyntic mucosa, in a pattern mirroring that of ghrelin immunoreactivity and ghrelin mRNA. hrGFP expression also was observed in the duodenum, but not in the brain, pancreatic islet, or testis. In addition, we used fluorescent activated cell sorting (FACS) to collect and partially characterize highly enriched populations of gastric ghrelin cells. We suggest that these novel ghrelin-hrGFP transgenic mice will serve as useful tools to better understand ghrelin cell physiology.

Original language	English (US)
Pages (from-to)	91-98
Number of pages	8
Journal	Regulatory Peptides
Volume	155
Issue number	1-3
DOIs	https://doi.org/10.1016/j.regpep.2009.04.001
State	Published - Jun 5 2009

Keywords

FACS
Ghrelin
Green fluorescence protein
Transgenic

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/j.regpep.2009.04.001

Cite this

@article{c857b352ab9d4f3988eb55b605ac654d,

title = "Characterization of a novel ghrelin cell reporter mouse",

abstract = "Ghrelin is a hormone that influences many physiological processes and behaviors, such as food intake, insulin and growth hormone release, and a coordinated response to chronic stress. However, little is known about the molecular pathways governing ghrelin release and ghrelin cell function. To better study ghrelin cell physiology, we have generated several transgenic mouse lines expressing humanized Renilla reniformis green fluorescent protein (hrGFP) under the control of the mouse ghrelin promoter. hrGFP expression was especially abundant in the gastric oxyntic mucosa, in a pattern mirroring that of ghrelin immunoreactivity and ghrelin mRNA. hrGFP expression also was observed in the duodenum, but not in the brain, pancreatic islet, or testis. In addition, we used fluorescent activated cell sorting (FACS) to collect and partially characterize highly enriched populations of gastric ghrelin cells. We suggest that these novel ghrelin-hrGFP transgenic mice will serve as useful tools to better understand ghrelin cell physiology.",

keywords = "FACS, Ghrelin, Green fluorescence protein, Transgenic",

author = "Ichiro Sakata and Yoshihide Nakano and Sherri Osborne-Lawrence and Rovinsky, {Sherry A.} and Lee, {Charlotte E.} and Mario Perello and Anderson, {Jason G.} and Roberto Coppari and Guanghua Xiao and Lowell, {Bradford B.} and Elmquist, {Joel K.} and Zigman, {Jeffrey M.}",

note = "Funding Information: We thank Dr. Joel Lawitts, director of the BIDMC Transgenic Facility, and Dr. Robert E. Hammer, director of the UTSW Transgenic Core Facility, for their assistance with the generation of the ghrelin-hrGFP mouse lines. We thank Syann Lee, Jen-Chieh Chuang, and Angie Bookout for many helpful discussions. This work was supported by funding from the NIH (1F32DK64564-01, K08DK068069-01A2, 5 P01 DK056116-09 and R01DK71320), Takeda Pharmaceutical Company, Ltd., and a University of Texas Southwestern Medical Center Disease-Oriented Clinical Scholars Award.",

year = "2009",

month = jun,

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doi = "10.1016/j.regpep.2009.04.001",

language = "English (US)",

volume = "155",

pages = "91--98",

journal = "Regulatory Peptides",

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T1 - Characterization of a novel ghrelin cell reporter mouse

AU - Sakata, Ichiro

AU - Nakano, Yoshihide

AU - Osborne-Lawrence, Sherri

AU - Rovinsky, Sherry A.

AU - Lee, Charlotte E.

AU - Perello, Mario

AU - Anderson, Jason G.

AU - Coppari, Roberto

AU - Xiao, Guanghua

AU - Lowell, Bradford B.

AU - Elmquist, Joel K.

AU - Zigman, Jeffrey M.

N1 - Funding Information: We thank Dr. Joel Lawitts, director of the BIDMC Transgenic Facility, and Dr. Robert E. Hammer, director of the UTSW Transgenic Core Facility, for their assistance with the generation of the ghrelin-hrGFP mouse lines. We thank Syann Lee, Jen-Chieh Chuang, and Angie Bookout for many helpful discussions. This work was supported by funding from the NIH (1F32DK64564-01, K08DK068069-01A2, 5 P01 DK056116-09 and R01DK71320), Takeda Pharmaceutical Company, Ltd., and a University of Texas Southwestern Medical Center Disease-Oriented Clinical Scholars Award.

PY - 2009/6/5

Y1 - 2009/6/5

N2 - Ghrelin is a hormone that influences many physiological processes and behaviors, such as food intake, insulin and growth hormone release, and a coordinated response to chronic stress. However, little is known about the molecular pathways governing ghrelin release and ghrelin cell function. To better study ghrelin cell physiology, we have generated several transgenic mouse lines expressing humanized Renilla reniformis green fluorescent protein (hrGFP) under the control of the mouse ghrelin promoter. hrGFP expression was especially abundant in the gastric oxyntic mucosa, in a pattern mirroring that of ghrelin immunoreactivity and ghrelin mRNA. hrGFP expression also was observed in the duodenum, but not in the brain, pancreatic islet, or testis. In addition, we used fluorescent activated cell sorting (FACS) to collect and partially characterize highly enriched populations of gastric ghrelin cells. We suggest that these novel ghrelin-hrGFP transgenic mice will serve as useful tools to better understand ghrelin cell physiology.

AB - Ghrelin is a hormone that influences many physiological processes and behaviors, such as food intake, insulin and growth hormone release, and a coordinated response to chronic stress. However, little is known about the molecular pathways governing ghrelin release and ghrelin cell function. To better study ghrelin cell physiology, we have generated several transgenic mouse lines expressing humanized Renilla reniformis green fluorescent protein (hrGFP) under the control of the mouse ghrelin promoter. hrGFP expression was especially abundant in the gastric oxyntic mucosa, in a pattern mirroring that of ghrelin immunoreactivity and ghrelin mRNA. hrGFP expression also was observed in the duodenum, but not in the brain, pancreatic islet, or testis. In addition, we used fluorescent activated cell sorting (FACS) to collect and partially characterize highly enriched populations of gastric ghrelin cells. We suggest that these novel ghrelin-hrGFP transgenic mice will serve as useful tools to better understand ghrelin cell physiology.

KW - FACS

KW - Ghrelin

KW - Green fluorescence protein

KW - Transgenic

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ER -

Characterization of a novel ghrelin cell reporter mouse

Abstract

Keywords

ASJC Scopus subject areas

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