Abstract
We have cloned the al-2 gene of Neurospora crassa and have analyzed its structure and regulation. The gene encodes a 603-residue polypeptide with a segment homologous to prokaryotic and other eukaryotic phytoene syntheses. RNA measurements showed that the level of al-2 mRNA increased over 30-fold in photoinduced mycelia compared with dark-grown mycelia. This observation is consistent with the fact that carotenoid biosynthesis is induced by blue light during growth of N. crassa mycelia. The photoinduced increase in al-2 mRNA levels was not observed in two Neurospora mutants, wc-1 and wc-2, that are defective in all physiological photoresponses.
Original language | English (US) |
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Pages (from-to) | 12060-12066 |
Number of pages | 7 |
Journal | Journal of Biological Chemistry |
Volume | 269 |
Issue number | 16 |
State | Published - Apr 22 1994 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology