Characterization of an animal model of vitamin B12 deprivation

E. P. Frenkel; J. D. White

Characterization of an animal model of vitamin B12 deprivation

E. P. Frenkel, J. D. White

Research output: Contribution to journal › Article › peer-review

Abstract

A reproducible model of vitamin B₁₂ deprivation was evaluated and the resultant sequential biochemical changes were characterized. Weanling rats, protected against coprophagy, were maintained on B₁₂ deprived diet which resulted in limited growth and maturation. Specificity of the deprivation was documented by correction with only the addition of parenteral vitamin B₁₂. During deprivation serum B₁₂ values declined first and then tissue levels fell. Biochemical intermediates of disordered B₁₂ coenzyme metabolism (propionic and methylmalonic aciduria) were variable in excretion. Documentation of deranged B₁₂ metabolism was obtained by evidence of defective propionate catabolism. The sequence of changes was characterized in this animal model of vitamin B₁₂ deprivation.

Original language	English (US)
Pages (from-to)	614-619
Number of pages	6
Journal	Laboratory Investigation
Volume	29
Issue number	6
State	Published - Dec 1 1973

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Cell Biology

Cite this

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abstract = "A reproducible model of vitamin B12 deprivation was evaluated and the resultant sequential biochemical changes were characterized. Weanling rats, protected against coprophagy, were maintained on B12 deprived diet which resulted in limited growth and maturation. Specificity of the deprivation was documented by correction with only the addition of parenteral vitamin B12. During deprivation serum B12 values declined first and then tissue levels fell. Biochemical intermediates of disordered B12 coenzyme metabolism (propionic and methylmalonic aciduria) were variable in excretion. Documentation of deranged B12 metabolism was obtained by evidence of defective propionate catabolism. The sequence of changes was characterized in this animal model of vitamin B12 deprivation.",

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N2 - A reproducible model of vitamin B12 deprivation was evaluated and the resultant sequential biochemical changes were characterized. Weanling rats, protected against coprophagy, were maintained on B12 deprived diet which resulted in limited growth and maturation. Specificity of the deprivation was documented by correction with only the addition of parenteral vitamin B12. During deprivation serum B12 values declined first and then tissue levels fell. Biochemical intermediates of disordered B12 coenzyme metabolism (propionic and methylmalonic aciduria) were variable in excretion. Documentation of deranged B12 metabolism was obtained by evidence of defective propionate catabolism. The sequence of changes was characterized in this animal model of vitamin B12 deprivation.

AB - A reproducible model of vitamin B12 deprivation was evaluated and the resultant sequential biochemical changes were characterized. Weanling rats, protected against coprophagy, were maintained on B12 deprived diet which resulted in limited growth and maturation. Specificity of the deprivation was documented by correction with only the addition of parenteral vitamin B12. During deprivation serum B12 values declined first and then tissue levels fell. Biochemical intermediates of disordered B12 coenzyme metabolism (propionic and methylmalonic aciduria) were variable in excretion. Documentation of deranged B12 metabolism was obtained by evidence of defective propionate catabolism. The sequence of changes was characterized in this animal model of vitamin B12 deprivation.

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Characterization of an animal model of vitamin B12 deprivation

Abstract

ASJC Scopus subject areas

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