A reproducible model of vitamin B12 deprivation was evaluated and the resultant sequential biochemical changes were characterized. Weanling rats, protected against coprophagy, were maintained on B12 deprived diet which resulted in limited growth and maturation. Specificity of the deprivation was documented by correction with only the addition of parenteral vitamin B12. During deprivation serum B12 values declined first and then tissue levels fell. Biochemical intermediates of disordered B12 coenzyme metabolism (propionic and methylmalonic aciduria) were variable in excretion. Documentation of deranged B12 metabolism was obtained by evidence of defective propionate catabolism. The sequence of changes was characterized in this animal model of vitamin B12 deprivation.
|Original language||English (US)|
|Number of pages||6|
|State||Published - Dec 1 1973|
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Molecular Biology
- Cell Biology