Characterization of ataxia telangiectasia fibroblasts with extended life-span through telomerase expression

Lauren D. Wood, Tanya L. Halvorsen, Sonu Dhar, Joseph A. Baur, Raj K. Pandita, Woodring E. Wright, M. Prakash Hande, Gloria Calaf, Tom K. Hei, Fred Levine, Jerry W. Shay, Jean J Y Wang, Tej K. Pandita

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

Ataxia-telangiectasia (A-T) is an autosomal recessive disease characterized by progressive cerebellar degeneration, immunodeficiencies, genomic instability and gonadal atrophy. A-T patients are hypersensitive to ionizing radiation and have an elevated cancer risk. Cells derived from A-T patients require higher levels of serum factors, exhibit cytoskeletal defects and undergo premature senescence in culture. We show here that expression of the catalytic subunit of telomerase (hTERT) in primary A-T patient fibroblasts can rescue the premature senescence phenotype. Ectopic expression of hTERT does not rescue the radiosensitivity or the telomere fusions in A-T fibroblasts. The hTERT + AT cells also retain the characteristic defects in cell-cycle checkpoints, and show increased chromosome damage before and after ionizing radiation. Although A-T patients have an increased susceptibility to cancer, the expression of hTERT in A-T fibroblasts does not stimulate malignant transformation. These immortalized A-T cells provide a more stable cell system to investigate the molecular mechanisms underlying the cellular phenotypes of Ataxia-telangiectasia.

Original languageEnglish (US)
Pages (from-to)278-288
Number of pages11
JournalOncogene
Volume20
Issue number3
DOIs
StatePublished - Jan 18 2001

Keywords

  • ATM
  • Immortal
  • Ionizing radiation
  • Senescence
  • hTERT

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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  • Cite this

    Wood, L. D., Halvorsen, T. L., Dhar, S., Baur, J. A., Pandita, R. K., Wright, W. E., Hande, M. P., Calaf, G., Hei, T. K., Levine, F., Shay, J. W., Wang, J. J. Y., & Pandita, T. K. (2001). Characterization of ataxia telangiectasia fibroblasts with extended life-span through telomerase expression. Oncogene, 20(3), 278-288. https://doi.org/10.1038/sj.onc.1204072