Characterization of B cell antibodies in kidney transplant recipients

G. Nunez, J. J. McPhaul, P. Stastny

Research output: Contribution to journalArticle

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Abstract

Antibodies against B lymphocytes were found in the serum of the majority of 59 kidney transplant recipients adn of 22 eluates obtained from kidney allografts undergoing rejection. To characterize these B cell lymphocytotoxins we have used a mouse monoclonal anti-DR antibody (L227) that inhibits cytotoxicity of antibodies against HLA-DR antigens and a chicken serum against human Ia-like antigens that also inhibits antibodies against DR-related supertypic determinants and other Class II histocompatibility antigens. Three types of B cell cytotoxins were defined: antibodies against HLA-DR, antibodies against Ia-like antigens other than DR, and antibodies against non-Ia-related B cell antigens. Before transplantation, B cell antibodies were detected in about a third of the patients studied. They were inhibited by monoclonal anti-DR more often in recipients who ultimately rejected a kidney allograft (67%) than in those in whom the graft was successful (44%, P < 0.03). After transplantation, antibodies inhibited by L227 were found in 56% of the patients with functioning grafts and in 94% of the recipients whose grafts had been removed because of rejection (P < 0.001). B cell antibodies inhibited by monoclonal anti-DR were found in the majority of kidney eluates. However, although 85% of the B cell reactions of kidney eluates were blocked by this antibody, only 55% of the B cell reactions of sera obtained from the same recipients after nephrectomy were similarly inhibited. Thus it appears that antibodies against HLA-DR were bound and concentrated in the transplanted organ and other B cell antibodies were not. These results indicate that anti-DR antibodies blocked by the monoclonal antibody L227 are the most common type of B cell lymphocytotoxins formed in kidney transplant recipients. Their role in kidney allografts undergoing rejection, where they are bound in high concentration, needs to be determined.

Original languageEnglish (US)
Pages (from-to)446-452
Number of pages7
JournalTransplantation
Volume35
Issue number5
StatePublished - 1983

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B-Lymphocytes
Kidney
Antibodies
HLA-DR Antigens
Allografts
Histocompatibility Antigens Class II
Monoclonal Antibodies
Transplants
Anti-Idiotypic Antibodies
Transplant Recipients
Transplantation
Serum
HLA-D Antigens
Cytotoxins
Nephrectomy
Chickens
Antigens

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Nunez, G., McPhaul, J. J., & Stastny, P. (1983). Characterization of B cell antibodies in kidney transplant recipients. Transplantation, 35(5), 446-452.

Characterization of B cell antibodies in kidney transplant recipients. / Nunez, G.; McPhaul, J. J.; Stastny, P.

In: Transplantation, Vol. 35, No. 5, 1983, p. 446-452.

Research output: Contribution to journalArticle

Nunez, G, McPhaul, JJ & Stastny, P 1983, 'Characterization of B cell antibodies in kidney transplant recipients', Transplantation, vol. 35, no. 5, pp. 446-452.
Nunez, G. ; McPhaul, J. J. ; Stastny, P. / Characterization of B cell antibodies in kidney transplant recipients. In: Transplantation. 1983 ; Vol. 35, No. 5. pp. 446-452.
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