Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter

Young Ah Moon, Sahng Wook Park, Kyung Sup Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The cis-acting element in the promoter of the rat ATP-citrate lyase (ACL) and transcription factors which interact with these elements were determined. Six Sp1 binding sites and CAAT box exist in the region from transcription start site to -419 bp which showed the highest promoter activity in ACL promoter previously. In the region from -612 to -419, C/EBP binding site and other protein binding sites were also detected. Chloramphenicol acetyltransferase assay of ACL promoter suggested that multiple Sp1 sites might be involved in the ACL transcription. Gel mobility shift assay with antibodies against Sp1 and Sp3 revealed that DNA binding efficiency of Sp1 is increased in the liver of rats re-fed low fat/high carbohydrate diet after fasting. These results suggest that Sp1 is one of the most important transcription factors for ACL promoter to produce basal and induced transcription by low fat/high carbohydrate diet.

Original languageEnglish (US)
Pages (from-to)60-68
Number of pages9
JournalExperimental and Molecular Medicine
Volume34
Issue number1
StatePublished - Mar 31 2002

Fingerprint

ATP Citrate (pro-S)-Lyase
Rats
Genes
Binding Sites
High Fat Diet
Transcription
Nutrition
Assays
Transcription Factors
Fats
Carbohydrates
Chloramphenicol O-Acetyltransferase
Transcription Initiation Site
Electrophoretic Mobility Shift Assay
Protein Binding
Liver
Fasting
Gels
Antibodies
DNA

Keywords

  • ATP citrate-lyase promoter
  • C/EBP
  • Lipogenesis
  • NF-Y
  • Sp1

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter. / Moon, Young Ah; Park, Sahng Wook; Kim, Kyung Sup.

In: Experimental and Molecular Medicine, Vol. 34, No. 1, 31.03.2002, p. 60-68.

Research output: Contribution to journalArticle

Moon, Young Ah ; Park, Sahng Wook ; Kim, Kyung Sup. / Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter. In: Experimental and Molecular Medicine. 2002 ; Vol. 34, No. 1. pp. 60-68.
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