Characterization of embryonic globin genes of the zebrafish

Alison Brownlie, Candace Hersey, Andrew C. Oates, Barry H. Paw, Arnold M. Falick, H. Ewa Witkowska, Jonathan Flint, Doug Higgs, Jason Jessen, Nathan Bahary, Hao Zhu, Shuo Lin, Leonard Zon

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Hemoglobin switching is a complex process by which distinct globin chains are produced during stages of development. In an effort to characterize the process of hemoglobin switching in the zebrafish model system, we have isolated and characterized several embryonic globin genes. The embryonic and adult globin genes are found in clusters in a head-to-head configuration. One cluster of embryonic and adult genes is localized to linkage group 3, whereas another embryonic cluster is localized on linkage group 12. Several embryonic globin genes demonstrate an erythroid-specific pattern of expression early during embryogenesis and later are downregulated as definitive hematopoiesis occurs. We utilized electrospray mass spectroscopy to correlate globin genes and protein expression in developing embryonic red cells. The mutation, zinfandel, has a hypochromic microcytic anemia as an embryo, but later recovers in adulthood. The zinfandel gene maps to linkage group 3 near the major globin gene locus, strongly suggesting that zinfandel represents an embryonic globin defect. Our studies are the first to systematically evaluate the embryonic globins in the zebrafish and will ultimately be useful in evaluating zebrafish mutants with defects in hemoglobin production and switching.

Original languageEnglish (US)
Pages (from-to)48-61
Number of pages14
JournalDevelopmental Biology
Volume255
Issue number1
DOIs
StatePublished - Mar 1 2003

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Globins
Zebrafish
Genes
Hemoglobins
Hematopoiesis
Embryonic Development
Mass Spectrometry
Down-Regulation
Embryonic Structures
Gene Expression
Mutation

Keywords

  • Globin
  • Hematopoiesis
  • Thalassemia
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Brownlie, A., Hersey, C., Oates, A. C., Paw, B. H., Falick, A. M., Witkowska, H. E., ... Zon, L. (2003). Characterization of embryonic globin genes of the zebrafish. Developmental Biology, 255(1), 48-61. https://doi.org/10.1016/S0012-1606(02)00041-6

Characterization of embryonic globin genes of the zebrafish. / Brownlie, Alison; Hersey, Candace; Oates, Andrew C.; Paw, Barry H.; Falick, Arnold M.; Witkowska, H. Ewa; Flint, Jonathan; Higgs, Doug; Jessen, Jason; Bahary, Nathan; Zhu, Hao; Lin, Shuo; Zon, Leonard.

In: Developmental Biology, Vol. 255, No. 1, 01.03.2003, p. 48-61.

Research output: Contribution to journalArticle

Brownlie, A, Hersey, C, Oates, AC, Paw, BH, Falick, AM, Witkowska, HE, Flint, J, Higgs, D, Jessen, J, Bahary, N, Zhu, H, Lin, S & Zon, L 2003, 'Characterization of embryonic globin genes of the zebrafish', Developmental Biology, vol. 255, no. 1, pp. 48-61. https://doi.org/10.1016/S0012-1606(02)00041-6
Brownlie A, Hersey C, Oates AC, Paw BH, Falick AM, Witkowska HE et al. Characterization of embryonic globin genes of the zebrafish. Developmental Biology. 2003 Mar 1;255(1):48-61. https://doi.org/10.1016/S0012-1606(02)00041-6
Brownlie, Alison ; Hersey, Candace ; Oates, Andrew C. ; Paw, Barry H. ; Falick, Arnold M. ; Witkowska, H. Ewa ; Flint, Jonathan ; Higgs, Doug ; Jessen, Jason ; Bahary, Nathan ; Zhu, Hao ; Lin, Shuo ; Zon, Leonard. / Characterization of embryonic globin genes of the zebrafish. In: Developmental Biology. 2003 ; Vol. 255, No. 1. pp. 48-61.
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