Characterization of fig operon mutants of Francisella novicida U112

Katalin Kiss, Wei Liu, Jason F. Huntley, Michael V. Norgard, Eric J. Hansen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Francisella species secrete a polycarboxylate siderophore that resembles rhizoferrin to acquire ferric iron. Several of the Francisella siderophore synthesis genes are contained in a Fur-regulated operon (designated fig or fsl) comprised of at least seven ORFs including fur. Reverse transcriptase-PCR showed transcriptional linkage between figD and figE and between figE and figF. Mutations were constructed in four of these ORFs (figB, figC, figD, and figE) in Francisella novicida U112. All four of these new mutants and a F. novicida figA mutant grew at rates comparable to that of wild type under iron-replete conditions but growth of all five mutants was stunted in iron-limiting media. When ferric rhizoferrin was added to the iron-limited media, growth of the figA, figB, figC, and figD mutants was restored to levels similar to those obtained in iron-replete media. However, this exogenously added siderophore could not rescue the figE mutant. When Chrome Azurol S assays were used to measure siderophore production, the figA, figB, and figC mutants were markedly deficient in their ability to synthesize siderophore whereas the figD and figE mutants produced siderophore at levels equivalent to the wild-type parent strain.

Original languageEnglish (US)
Pages (from-to)270-277
Number of pages8
JournalFEMS Microbiology Letters
Volume285
Issue number2
DOIs
StatePublished - Aug 2008

Fingerprint

Francisella
Ficus
Siderophores
Operon
Iron
Open Reading Frames
Growth
Reverse Transcriptase Polymerase Chain Reaction
Mutation

Keywords

  • Francisella
  • Iron
  • Mutant
  • Rhizoferrin
  • Siderophore

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Characterization of fig operon mutants of Francisella novicida U112. / Kiss, Katalin; Liu, Wei; Huntley, Jason F.; Norgard, Michael V.; Hansen, Eric J.

In: FEMS Microbiology Letters, Vol. 285, No. 2, 08.2008, p. 270-277.

Research output: Contribution to journalArticle

Kiss, Katalin ; Liu, Wei ; Huntley, Jason F. ; Norgard, Michael V. ; Hansen, Eric J. / Characterization of fig operon mutants of Francisella novicida U112. In: FEMS Microbiology Letters. 2008 ; Vol. 285, No. 2. pp. 270-277.
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