Characterization of Gastric and Neuronal Histaminergic Populations Using a Transgenic Mouse Model

Angela K. Walker, Won Mee Park, Jen Chieh Chuang, Mario Perello, Ichiro Sakata, Sherri Osborne-Lawrence, Jeffrey M. Zigman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Histamine is a potent biogenic amine that mediates numerous physiological processes throughout the body, including digestion, sleep, and immunity. It is synthesized by gastric enterochromaffin-like cells, a specific set of hypothalamic neurons, as well as a subset of white blood cells, including mast cells. Much remains to be learned about these varied histamine-producing cell populations. Here, we report the validation of a transgenic mouse line in which Cre recombinase expression has been targeted to cells expressing histidine decarboxylase (HDC), which catalyzes the rate-limiting step in the synthesis of histamine. This was achieved by crossing the HDC-Cre mouse line with Rosa26-tdTomato reporter mice, thus resulting in the expression of the fluorescent Tomato (Tmt) signal in cells containing Cre recombinase activity. As expected, the Tmt signal co-localized with HDC-immunoreactivity within the gastric mucosa and gastric submucosa and also within the tuberomamillary nucleus of the brain. HDC expression within Tmt-positive gastric cells was further confirmed by quantitative PCR analysis of mRNA isolated from highly purified populations of Tmt-positive cells obtained by fluorescent activated cell sorting (FACS). HDC expression within these FACS-separated cells was found to coincide with other markers of both ECL cells and mast cells. Gastrin expression was co-localized with HDC expression in a subset of histaminergic gastric mucosal cells. We suggest that these transgenic mice will facilitate future studies aimed at investigating the function of histamine-producing cells.

Original languageEnglish (US)
Article numbere60276
JournalPLoS One
Volume8
Issue number3
DOIs
StatePublished - Mar 29 2013

Fingerprint

Histidine Decarboxylase
Transgenic Mice
Stomach
stomach
animal models
histidine decarboxylase
genetically modified organisms
Histamine
Cells
Population
Lycopersicon esculentum
cells
histamine
Sorting
Enterochromaffin-like Cells
tomatoes
Emitter coupled logic circuits
Biogenic Amines
Mast Cells
Gastrins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Walker, A. K., Park, W. M., Chuang, J. C., Perello, M., Sakata, I., Osborne-Lawrence, S., & Zigman, J. M. (2013). Characterization of Gastric and Neuronal Histaminergic Populations Using a Transgenic Mouse Model. PLoS One, 8(3), [e60276]. https://doi.org/10.1371/journal.pone.0060276

Characterization of Gastric and Neuronal Histaminergic Populations Using a Transgenic Mouse Model. / Walker, Angela K.; Park, Won Mee; Chuang, Jen Chieh; Perello, Mario; Sakata, Ichiro; Osborne-Lawrence, Sherri; Zigman, Jeffrey M.

In: PLoS One, Vol. 8, No. 3, e60276, 29.03.2013.

Research output: Contribution to journalArticle

Walker, AK, Park, WM, Chuang, JC, Perello, M, Sakata, I, Osborne-Lawrence, S & Zigman, JM 2013, 'Characterization of Gastric and Neuronal Histaminergic Populations Using a Transgenic Mouse Model', PLoS One, vol. 8, no. 3, e60276. https://doi.org/10.1371/journal.pone.0060276
Walker AK, Park WM, Chuang JC, Perello M, Sakata I, Osborne-Lawrence S et al. Characterization of Gastric and Neuronal Histaminergic Populations Using a Transgenic Mouse Model. PLoS One. 2013 Mar 29;8(3). e60276. https://doi.org/10.1371/journal.pone.0060276
Walker, Angela K. ; Park, Won Mee ; Chuang, Jen Chieh ; Perello, Mario ; Sakata, Ichiro ; Osborne-Lawrence, Sherri ; Zigman, Jeffrey M. / Characterization of Gastric and Neuronal Histaminergic Populations Using a Transgenic Mouse Model. In: PLoS One. 2013 ; Vol. 8, No. 3.
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