Characterization of glucose transport system in Drosophila Kc cells

May yun Wang, Chung Wang

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We have characterized the glucose transport system in the Kc cells of Drosophila melanogaster. The transporter is stereospecific and can be inhibited by both cytochalasin B and phloretin, with ki, values of 4 μM and 30 μM for cytochalasin B and phloretin, respectively. Moreover, phloridzin does not affect uptake of glucose by Kc cells. Thus, on the basis of inhibitor studies, it appears that this insect cell line contains glucose transporters similar to those of the mammalian cells. However, probes prepared from rat transporter cDNAs showed no cross-hybridization with Drosophila RNA under moderately stringent conditions. Sugar selectivity has also been investigated by competition assay. The km values for d-glucose, 2-deoxy-d-glucose, d-fructose and d-galactose are 2 mM, 6 mM, 17 mM and 16 mM, respectively. The results indicate that this transporter prefers d-glucose and that it does not discriminate between d-galactose and d-fructose.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalFEBS Letters
Volume317
Issue number3
DOIs
StatePublished - Feb 15 1993

Keywords

  • Drosophila melanogaster
  • Glucose transporter
  • Kc cell

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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