Characterization of glucose transport system in Drosophila Kc cells

May yun Wang, Chung Wang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have characterized the glucose transport system in the Kc cells of Drosophila melanogaster. The transporter is stereospecific and can be inhibited by both cytochalasin B and phloretin, with ki, values of 4 μM and 30 μM for cytochalasin B and phloretin, respectively. Moreover, phloridzin does not affect uptake of glucose by Kc cells. Thus, on the basis of inhibitor studies, it appears that this insect cell line contains glucose transporters similar to those of the mammalian cells. However, probes prepared from rat transporter cDNAs showed no cross-hybridization with Drosophila RNA under moderately stringent conditions. Sugar selectivity has also been investigated by competition assay. The km values for d-glucose, 2-deoxy-d-glucose, d-fructose and d-galactose are 2 mM, 6 mM, 17 mM and 16 mM, respectively. The results indicate that this transporter prefers d-glucose and that it does not discriminate between d-galactose and d-fructose.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalFEBS Letters
Volume317
Issue number3
DOIs
StatePublished - Feb 15 1993

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Drosophila
Glucose
Phloretin
Cytochalasin B
Fructose
Galactose
Cells
Phlorhizin
Facilitative Glucose Transport Proteins
Drosophila melanogaster
Sugars
Insects
Rats
Assays
Complementary DNA
RNA
Cell Line

Keywords

  • Drosophila melanogaster
  • Glucose transporter
  • Kc cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterization of glucose transport system in Drosophila Kc cells. / Wang, May yun; Wang, Chung.

In: FEBS Letters, Vol. 317, No. 3, 15.02.1993, p. 241-244.

Research output: Contribution to journalArticle

Wang, May yun ; Wang, Chung. / Characterization of glucose transport system in Drosophila Kc cells. In: FEBS Letters. 1993 ; Vol. 317, No. 3. pp. 241-244.
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