Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays

Jun Li, Wei Zhao, Rehan Akbani, Wenbin Liu, Zhenlin Ju, Shiyun Ling, Christopher P. Vellano, Paul Roebuck, Qinghua Yu, A. Karina Eterovic, Lauren A. Byers, Michael A. Davies, Wanleng Deng, Y. N Vashisht Gopal, Guo Chen, Erika M. von Euw, Dennis Slamon, Dylan Conklin, John V. Heymach, Adi F. GazdarJohn D. Minna, Jeffrey N. Myers, Yiling Lu, Gordon B. Mills, Han Liang

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Cancer cell lines are major model systems for mechanistic investigation and drug development. However, protein expression data linked to high-quality DNA, RNA, and drug-screening data have not been available across a large number of cancer cell lines. Using reverse-phase protein arrays, we measured expression levels of ∼230 key cancer-related proteins in >650 independent cell lines, many of which have publically available genomic, transcriptomic, and drug-screening data. Our dataset recapitulates the effects of mutated pathways on protein expression observed in patient samples, and demonstrates that proteins and particularly phosphoproteins provide information for predicting drug sensitivity that is not available from the corresponding mRNAs. We also developed a user-friendly bioinformatic resource, MCLP, to help serve the biomedical research community.

Original languageEnglish (US)
Pages (from-to)225-239
Number of pages15
JournalCancer Cell
Volume31
Issue number2
DOIs
StatePublished - Feb 13 2017

Keywords

  • biomarker
  • cancer cell lines
  • data portal
  • drug sensitivity
  • proteomics
  • reverse-phase protein array
  • signaling pathways

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

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  • Cite this

    Li, J., Zhao, W., Akbani, R., Liu, W., Ju, Z., Ling, S., Vellano, C. P., Roebuck, P., Yu, Q., Eterovic, A. K., Byers, L. A., Davies, M. A., Deng, W., Gopal, Y. N. V., Chen, G., von Euw, E. M., Slamon, D., Conklin, D., Heymach, J. V., ... Liang, H. (2017). Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays. Cancer Cell, 31(2), 225-239. https://doi.org/10.1016/j.ccell.2017.01.005