Characterization of lymphangiogenesis in a model of adult skin regeneration

Joseph M. Rutkowski, Kendrick C. Boardman, Melody A. Swartz

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

To date, adult lymphangiogenesis is not well understood. In this study we describe the evolution of lymphatic capillaries in regenerating skin and correlate lymphatic migration and organization with the expression of matrix metalloproteinases (MMPs), immune cells, the growth factors VEGF-A and VEGF-C, and the heparan sulfate proteogylcan perlecan, a key component of basement membrane. We show that while lymphatic endothelial cells (LECs) migrate and organize unidirectionally, in the direction of interstitial fluid flow, they do not sprout into the region but rather migrate as single cells that later join together into vessels. Furthermore, in a modified "shunted flow" version of the model, infiltrated LECs fail to organize into functional vessels, indicating that interstitial fluid flow is necessary for lymphatic organization. Perlecan expression on new lymphatic vessels was only observed after vessel organization was complete and also appeared first in the distal region, consistent with the directionality of lymphatic migration and organization. VEGF-C expression peaked at the initiation of lymphangiogenesis but was reduced to lower levels throughout organization and maturation. In mice lacking MMP-9, lymphatics regenerated normally, suggesting that MMP-9 is not required for lymphangiogenesis, at least in mouse skin. This study thus characterizes the process of adult lymphangiogenesis and differentiates it from sprouting blood angiogenesis, verifies its dependence on interstitial fluid flow for vessel organization, and correlates its temporal evolution with those of relevant environmental factors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume291
Issue number3
DOIs
StatePublished - 2006

Fingerprint

Lymphangiogenesis
Regeneration
Extracellular Fluid
Vascular Endothelial Growth Factor C
Skin
Lymphatic Vessels
Matrix Metalloproteinase 9
Endothelial Cells
Heparitin Sulfate
Matrix Metalloproteinases
Basement Membrane
Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
perlecan

Keywords

  • Interstitial fluid flow
  • Lymphatic
  • Matrix metalloproteinase-9
  • Perlecan
  • Vasculogenesis

ASJC Scopus subject areas

  • Physiology

Cite this

Characterization of lymphangiogenesis in a model of adult skin regeneration. / Rutkowski, Joseph M.; Boardman, Kendrick C.; Swartz, Melody A.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 291, No. 3, 2006.

Research output: Contribution to journalArticle

Rutkowski, Joseph M. ; Boardman, Kendrick C. ; Swartz, Melody A. / Characterization of lymphangiogenesis in a model of adult skin regeneration. In: American Journal of Physiology - Heart and Circulatory Physiology. 2006 ; Vol. 291, No. 3.
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