Characterization of mesenchymal stem cells from human vocal fold fibroblasts

Summer E. Hanson, Jaehyup Kim, Beatriz H. Quinchia Johnson, Bridget Bradley, Melissa J. Breunig, Peiman Hematti, Susan L. Thibeault

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Objectives/Hypothesis: Mesenchymal stem cells (MSCs) originally isolated from bone marrow (BM), are fibroblast-looking cells that are now assumed to be present in the stromal component of many tissues. MSCs are characterized by a certain set of criteria, including their growth culture characteristics, a combination of cell surface markers, and the ability to differentiate along multiple mesenchymal tissue lineages. We hypothesized that human vocal fold fibroblasts (hVFF) isolated from the lamina propria meet the criteria established to define MSCs and are functionally similar to MSCs derived from BM and adipose tissue. Study Design: In vitro study. Methods: hVFF were previously derived from human vocal fold tissues. MSCs were derived from adipose tissue (AT), and BM of healthy donors based on their attachment to culture dishes and their morphology and expanded in culture. Cells were analyzed for standard cell surface markers identified on BM-derived MSCs and the ability to differentiate into cells of mesenchymal lineage (i.e., fat, bone, and cartilage). We investigated the immunophenotype of these cells before and after interferon-γ (INF-γ) stimulation. Results: hVFF displayed cell surface markers and multipotent differentiation capacity characteristic of MSCs. Furthermore, these cells exhibited similar patterns of expression of human leukocyte antigen and costimulatory molecules, after stimulation with INF-c compared to MSCs derived from BM and AT. Conclusions: Based on our findings, hVFF derived from lamina propria have the same cell surface markers, immunophenotypic characteristics, and differentiation potential as BM- and AT-derived MSCs. We propose that vocal fold fibroblasts are MSCs resident in the vocal fold lamina propria.

Original languageEnglish (US)
Pages (from-to)546-551
Number of pages6
JournalLaryngoscope
Volume120
Issue number3
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

Fingerprint

Vocal Cords
Mesenchymal Stromal Cells
Fibroblasts
Bone Marrow
Adipose Tissue
Bone and Bones
Mucous Membrane
Interferons
Differentiation Antigens
Cell Lineage
HLA Antigens
Cartilage
Fats

Keywords

  • Human vocal fold stem cells
  • Immune modulation
  • Mesenchymal stem cells
  • Vocal fold fibroblasts

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Hanson, S. E., Kim, J., Quinchia Johnson, B. H., Bradley, B., Breunig, M. J., Hematti, P., & Thibeault, S. L. (2010). Characterization of mesenchymal stem cells from human vocal fold fibroblasts. Laryngoscope, 120(3), 546-551. https://doi.org/10.1002/lary.20797

Characterization of mesenchymal stem cells from human vocal fold fibroblasts. / Hanson, Summer E.; Kim, Jaehyup; Quinchia Johnson, Beatriz H.; Bradley, Bridget; Breunig, Melissa J.; Hematti, Peiman; Thibeault, Susan L.

In: Laryngoscope, Vol. 120, No. 3, 01.03.2010, p. 546-551.

Research output: Contribution to journalArticle

Hanson, SE, Kim, J, Quinchia Johnson, BH, Bradley, B, Breunig, MJ, Hematti, P & Thibeault, SL 2010, 'Characterization of mesenchymal stem cells from human vocal fold fibroblasts', Laryngoscope, vol. 120, no. 3, pp. 546-551. https://doi.org/10.1002/lary.20797
Hanson SE, Kim J, Quinchia Johnson BH, Bradley B, Breunig MJ, Hematti P et al. Characterization of mesenchymal stem cells from human vocal fold fibroblasts. Laryngoscope. 2010 Mar 1;120(3):546-551. https://doi.org/10.1002/lary.20797
Hanson, Summer E. ; Kim, Jaehyup ; Quinchia Johnson, Beatriz H. ; Bradley, Bridget ; Breunig, Melissa J. ; Hematti, Peiman ; Thibeault, Susan L. / Characterization of mesenchymal stem cells from human vocal fold fibroblasts. In: Laryngoscope. 2010 ; Vol. 120, No. 3. pp. 546-551.
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