Characterization of monocot and dicot plant S-adenosyl-L-methionine decarboxylase gene families including identification in the mRNA of a highly conserved pair of upstream overlapping open reading frames

M. Franceschetti, C. Hanfrey, S. Scaramagli, P. Torrigiani, N. Bagni, D. Burtin, A. J. Michael

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

S-Adenosyl-L-methionine decarboxylase (AdoMetDC; EC 4.1.1.50) is one of the key regulatory enzymes in the biosynthesis of polyamines. Isolation of genomic and cDNA sequences from rice and Arabidopsis had indicated that this enzyme is encoded by a small multigene family in monocot and dicot plants. Analysis of rice, maize and Arabidopsis AdoMetDC cDNA species revealed that the monocot enzyme possesses an extended C-terminus relative to dicot and human enzymes. Interestingly, we discovered that all expressed plant AdoMetDC mRNA 5′ leader sequences contain a highly conserved pair of overlapping upstream open reading frames (uORFs) that overlap by one base. The 5′ tiny uORF consists of two or three codons and the 3′ small uORF encodes 50-54 residues. Sequences of the small uORFs are highly conserved between monocot, dicot and gymnosperm AdoMetDC mRNA species and the C-terminus of the plant small uORFs is conserved with the C-terminus of nematode AdoMetDC uORFs; such a conserved arrangement is strongly suggestive of a translational regulatory mechanism. No introns were found in the main AdoMetDC proenzyme ORF from any of the plant genes encoding AdoMetDC, whereas introns were found in conserved positions flanking the overlapping uORFs. The absence of the furthest 3′ intron from the Arabidopsis gene encoding AdoMetDC2 suggests that this intron was lost recently. Reverse-transcriptase-mediated PCR analysis of the two Arabidopsis genes for AdoMetDC indicated that AdoMetDC1 is abundant and ubiquitous, whereas the gene for AdoMetDC2 is expressed preferentially in leaves and inflorescences. Investigation of recently released Arabidopsis genome sequences has revealed that in addition to the two genes encoding AdoMetDC isolated as part of the present work, four additional genes are present in Arabidopsis but they are probably-not expressed.

Original languageEnglish (US)
Pages (from-to)403-409
Number of pages7
JournalBiochemical Journal
Volume353
Issue number2
DOIs
StatePublished - Jan 15 2001

Fingerprint

S-Adenosylmethionine
Introns
Open Reading Frames
Gene encoding
Genes
Arabidopsis
Messenger RNA
Enzymes
Complementary DNA
Enzyme Precursors
RNA-Directed DNA Polymerase
Biosynthesis
Polyamines
Gymnosperms
Inflorescence
Plant Genes
methionine decarboxylase
5' Untranslated Regions
Multigene Family
Reverse Transcriptase Polymerase Chain Reaction

Keywords

  • Polyamine
  • Translational regulation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of monocot and dicot plant S-adenosyl-L-methionine decarboxylase gene families including identification in the mRNA of a highly conserved pair of upstream overlapping open reading frames. / Franceschetti, M.; Hanfrey, C.; Scaramagli, S.; Torrigiani, P.; Bagni, N.; Burtin, D.; Michael, A. J.

In: Biochemical Journal, Vol. 353, No. 2, 15.01.2001, p. 403-409.

Research output: Contribution to journalArticle

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