Characterization of the chicken rhodopsin promoter: Identification of retina-specific and glass-like protein binding domains

Hooshmand Sheshberadaran, Joseph S. Takahashi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The rhodopsin promoter was analyzed as a prototype for cis-acting DNA elements involved in photoreceptor-specific gene regulation. We report the isolation and sequence of 1.8 kb of 5' flanking sequence of the chicken rhodopsin gene and analysis of DNA-binding proteins to promoter fragments by gel retardation assay. These analyses identified retina- and brain-specific protein binding to several regions of the rhodopsin promoter both proximal and distal to the transcription start site. The tissue-specific protein binding patterns were consistent with that proposed previously based on the expression pattern of rhodopsin promoter-lacZ constructs in transgenic mice (1, 2). The data also confirm the presence of the chicken homolog to a rat retina-specific binding site in the proximal promoter described previously. The Drosophila glass gene, which encodes a site-specific DNA-binding zinc- finger protein, is known to be required for the normal development of all photoreceptors in this species (3). In this paper we identify a glass-like sequence motif in chick, which shows homology to the Drosophila Rh1 27-bp glass-binding sequence, and find that the chick glass-like element exhibits tissue-specific protein binding properties which parallel those observed with Drosophila glass.

Original languageEnglish (US)
Pages (from-to)309-318
Number of pages10
JournalMolecular and Cellular Neurosciences
Volume5
Issue number4
DOIs
StatePublished - 1994

Fingerprint

Rhodopsin
Protein Binding
Glass
Retina
Chickens
Drosophila
Genes
5' Flanking Region
Transcription Initiation Site
DNA
Zinc Fingers
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
Genetic Promoter Regions
Transgenic Mice
Binding Sites
Protein Domains
Brain

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

@article{f67754658ce440048d1e862a1222fe15,
title = "Characterization of the chicken rhodopsin promoter: Identification of retina-specific and glass-like protein binding domains",
abstract = "The rhodopsin promoter was analyzed as a prototype for cis-acting DNA elements involved in photoreceptor-specific gene regulation. We report the isolation and sequence of 1.8 kb of 5' flanking sequence of the chicken rhodopsin gene and analysis of DNA-binding proteins to promoter fragments by gel retardation assay. These analyses identified retina- and brain-specific protein binding to several regions of the rhodopsin promoter both proximal and distal to the transcription start site. The tissue-specific protein binding patterns were consistent with that proposed previously based on the expression pattern of rhodopsin promoter-lacZ constructs in transgenic mice (1, 2). The data also confirm the presence of the chicken homolog to a rat retina-specific binding site in the proximal promoter described previously. The Drosophila glass gene, which encodes a site-specific DNA-binding zinc- finger protein, is known to be required for the normal development of all photoreceptors in this species (3). In this paper we identify a glass-like sequence motif in chick, which shows homology to the Drosophila Rh1 27-bp glass-binding sequence, and find that the chick glass-like element exhibits tissue-specific protein binding properties which parallel those observed with Drosophila glass.",
author = "Hooshmand Sheshberadaran and Takahashi, {Joseph S.}",
year = "1994",
doi = "10.1006/mcne.1994.1037",
language = "English (US)",
volume = "5",
pages = "309--318",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Characterization of the chicken rhodopsin promoter

T2 - Identification of retina-specific and glass-like protein binding domains

AU - Sheshberadaran, Hooshmand

AU - Takahashi, Joseph S.

PY - 1994

Y1 - 1994

N2 - The rhodopsin promoter was analyzed as a prototype for cis-acting DNA elements involved in photoreceptor-specific gene regulation. We report the isolation and sequence of 1.8 kb of 5' flanking sequence of the chicken rhodopsin gene and analysis of DNA-binding proteins to promoter fragments by gel retardation assay. These analyses identified retina- and brain-specific protein binding to several regions of the rhodopsin promoter both proximal and distal to the transcription start site. The tissue-specific protein binding patterns were consistent with that proposed previously based on the expression pattern of rhodopsin promoter-lacZ constructs in transgenic mice (1, 2). The data also confirm the presence of the chicken homolog to a rat retina-specific binding site in the proximal promoter described previously. The Drosophila glass gene, which encodes a site-specific DNA-binding zinc- finger protein, is known to be required for the normal development of all photoreceptors in this species (3). In this paper we identify a glass-like sequence motif in chick, which shows homology to the Drosophila Rh1 27-bp glass-binding sequence, and find that the chick glass-like element exhibits tissue-specific protein binding properties which parallel those observed with Drosophila glass.

AB - The rhodopsin promoter was analyzed as a prototype for cis-acting DNA elements involved in photoreceptor-specific gene regulation. We report the isolation and sequence of 1.8 kb of 5' flanking sequence of the chicken rhodopsin gene and analysis of DNA-binding proteins to promoter fragments by gel retardation assay. These analyses identified retina- and brain-specific protein binding to several regions of the rhodopsin promoter both proximal and distal to the transcription start site. The tissue-specific protein binding patterns were consistent with that proposed previously based on the expression pattern of rhodopsin promoter-lacZ constructs in transgenic mice (1, 2). The data also confirm the presence of the chicken homolog to a rat retina-specific binding site in the proximal promoter described previously. The Drosophila glass gene, which encodes a site-specific DNA-binding zinc- finger protein, is known to be required for the normal development of all photoreceptors in this species (3). In this paper we identify a glass-like sequence motif in chick, which shows homology to the Drosophila Rh1 27-bp glass-binding sequence, and find that the chick glass-like element exhibits tissue-specific protein binding properties which parallel those observed with Drosophila glass.

UR - http://www.scopus.com/inward/record.url?scp=0028133447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028133447&partnerID=8YFLogxK

U2 - 10.1006/mcne.1994.1037

DO - 10.1006/mcne.1994.1037

M3 - Article

C2 - 7804600

AN - SCOPUS:0028133447

VL - 5

SP - 309

EP - 318

JO - Molecular and Cellular Neuroscience

JF - Molecular and Cellular Neuroscience

SN - 1044-7431

IS - 4

ER -