Characterization of the CpxRA regulon in Haemophilus ducreyi

Research output: Contribution to journalArticle

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Abstract

The Haemophilus ducreyi 35000HP genome encodes a homolog of the CpxRA two-component cell envelope stress response system originally characterized in Escherichia coli. CpxR, the cytoplasmic response regulator, was shown previously to be involved in repression of the expression of the lspB-lspA2 operon (M. Labandeira-Rey, J. R. Mock, and E. J. Hansen, Infect. Immun. 77:3402-3411, 2009). In the present study, the H. ducreyi CpxR and CpxA proteins were shown to closely resemble those of other well-studied bacterial species. A cpxA deletion mutant and a CpxR-overexpressing strain were used to explore the extent of the CpxRA regulon. DNA microarray and real-time reverse transcriptase (RT) PCR analyses indicated several potential regulatory targets for the H. ducreyi CpxRA two-component regulatory system. Electrophoretic mobility shift assays (EMSAs) were used to prove that H. ducreyi CpxR interacted with the promoter regions of genes encoding both known and putative virulence factors of H. ducreyi, including the lspB-lspA2 operon, the flp operon, and dsrA. Interestingly, the use of EMSAs also indicated that H. ducreyi CpxR did not bind to the promoter regions of several genes predicted to encode factors involved in the cell envelope stress response. Taken together, these data suggest that the CpxRA system in H. ducreyi, in contrast to that in E. coli, may be involved primarily in controlling expression of genes not involved in the cell envelope stress response.

Original languageEnglish (US)
Pages (from-to)4779-4791
Number of pages13
JournalInfection and Immunity
Volume78
Issue number11
DOIs
StatePublished - Nov 2010

Fingerprint

Haemophilus ducreyi
Regulon
Operon
Electrophoretic Mobility Shift Assay
Genetic Promoter Regions
Escherichia coli
Complement Factor H
Virulence Factors
Cellular Structures
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Genes
Real-Time Polymerase Chain Reaction
Genome
Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Characterization of the CpxRA regulon in Haemophilus ducreyi. / Labandeira, Maria; Brautigam, Chad A; Hansen, Eric J.

In: Infection and Immunity, Vol. 78, No. 11, 11.2010, p. 4779-4791.

Research output: Contribution to journalArticle

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